Genetic polymorphism of CYP2D6∗2 C→T 2850, GSTM1, NQO1 genes and their correlation with biomarkers in manganese miners of Central India.

Manganese (Mn) intoxication is most often regarded as an occupational manifestation and occurs in places such as manganese mines, dry cell battery plants and ceramic industries. In the present study, the influence of genetic polymorphism in cytochrome P450 2D6 (CYP2D6∗2), glutathione S-transferase M1 (GSTM1) and NAD(P)H quinone oxidoreductase 1 (NQO1) genes on blood manganese and plasma prolactin concentrations in manganese miners was investigated. Genotyping of CYP2D6∗2 C→T 2850 and NQO1 C→T 609 was carried out using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) whereas the genotyping of GSTM1 was carried out by multiplex PCR using beta globin as an internal control.

Effect of organic forms of phosphorus and variable concentrations of sulfide on the metabolic generation of soluble-reactive phosphate by sulfur chemolithoautotrophs: a laboratory study.

In aquatic systems, recycling of phosphorus (P) is strongly influenced by microbial metabolic responses. This study was designed to look at the generation of P (as soluble-reactive phosphate (SRP)) from organic P-enriched sediments by benthic sulfur chemolithoautotrophs. The study revealed that microbial density was greater in organic P-enriched sediments when compared to sediments without added organic P and the presence of organic P significantly influenced mobilization of SRP from sediment.

Heavy metal status and oxidative stress in diesel engine tuning workers of central Indian population.

Objective: To assess the oxidative stress induced due to heavy metal exposure. Exposed populations are selected from an engine tuning station and control from the same area with no occupational exposure.

Method: Standard methods were followed for enzymatic assay, and heavy metals in blood and urine were analyzed by using inductively coupled plasma-optical emission spectrophotometer after microwave digestion.

Distribution of detoxifying genes polymorphism in Maharastrian population of central India.

The present study is aimed at evaluating the genotype frequency of detoxifying genes such as GSTM1, GSTT1 and NQO1 in Maharastrian population of central India. The study revealed about 64.6% of GSTM1-positive and 35.