Publications by authors named "Rajat Mann"
Article Synopsis
- Transcription factors like estrogen receptor α (ERα) regulate gene expression by binding to DNA and can interact with RNA, which may influence their binding to chromatin.
- This study found that RNA binding is crucial for maintaining proper recruitment of ERα to DNA, especially at weaker binding sites, and without this RNA interaction, ERα becomes more mobile in the nucleus.
- Increased mobility of ERα leads to enhanced transcription of genes with low-strength motifs, but stable binding at stronger sites may hinder ligand-dependent gene expression, suggesting RNA interactions help balance transcription regulation.
Transcription Factor (TF) condensates are a heterogenous mix of RNA, DNA, and multiple co-factor proteins capable of modulating the transcriptional response of the cell. The dynamic nature and the spatial location of TF-condensates in the 3D nuclear space is believed to provide a fast response, which is on the same pace as the signaling cascade and yet ever-so-specific in the crowded environment of the nucleus. However, the current understanding of how TF-condensates can achieve these feet so quickly and efficiently is still unclear.
View Article and Find Full Text PDFGenetic variation at the 8q24 locus is linked with the greater susceptibility to prostate cancer in men of African ancestry. One such African ancestry specific rare variant, rs72725854 (A>G/T) (~6% allele frequency) has been associated with a ~2-fold increase in prostate cancer risk. However, the functional relevance of this variant is unknown.
View Article and Find Full Text PDFUnliganded Estrogen receptor alpha (ERα) has been implicated in ligand-dependent gene regulation. Upon ligand exposure, ERα binds to several EREs relatively proximal to the pre-marked, unliganded ERα-bound sites and affects transient but robust gene expression. However, the underlying mechanisms are not fully understood.
View Article and Find Full Text PDF