Development of a calcium phosphate nanocomposite for fast fluorogenic detection of bacteria.

Current procedures for the detection and identification of bacterial infections are laborious, time-consuming, and require a high workload and well-equipped laboratories. Therefore the work presented herein developed a simple, fast, and low cost method for bacterial detection based on hydroxyapatite nanoparticles with a nutritive mixture and the fluorogenic substrate. Calcium phosphate ceramic nanoparticles were characterized and integrated with a nutritive mixture for the early detection of bacteria by visual as well as fluorescence spectroscopy techniques.

Efficacy of coral-hydroxyapatite and biphasic calcium phosphate for early bacterial detection.

Nano- or microhydroxyapatites with microbiological properties are being used to detect pathogens in clinical samples and industrial environments. In this study, the calcium phosphates coral-hydroxyapatite and biphasic calcium phosphate were characterized physicochemically using x-ray diffraction, thermogravimetric, and differential thermal analysis. The morphology, texture, and chemical composition of the ceramics were also investigated using scanning electron microscopy with energy dispersive spectroscopy.

[Assessment of the CromoCen ENT chromogenic medium for clinical diagnosis of Enterococcus species].

Introduction: The frequent incidence of Enterococci at hospitals and their growing antimicrobial resistance worldwide make the in-hospital surveillance and control a pressing need; consequently, it is indispensable to avail of more sensitive and accurate diagnostic means.

Objective: To broaden the evaluation of functionality of CromoCen ENT chromogenic medium for the isolation and identification of Enterococcus spp. from clinical samples.

[Identification of Candida species of clinical importance by means of a modified auxonographic method].

Introduction: Candida species are responsible for the most common opportunistic mycotic infections. Rapid and accurate identification of yeast has become relevant not only for the effective management of infections, but also for the prevention of drug resistance. Up to now, yeast identification procedures are based on a variety of tests, including their ability to use sugars.

[Characterization of Ipomoea batatas extract to be used as nutrient basis for culture media].

An Ipomoea batatas extract obtained by enzymatic hydrolysis with alpha amylase was characterized to be used as nutrient basis. Among its quality indicators, it had a content of over 50% whole carbohydrates with respect to the nominal mass, estimated by phenol-sulphur method. The same content of aminonitrogen detected and quantified by potentiometric titration using formaldehyde and of total nitrogen (0,23%) by Kjeldahl method were detected.

[Characterization of soy peptone for the culture of microorganisms].

A soy peptone obtained with enzyme papain as a hydrolyzing agent was characterized. The physicochemical evaluation of the nutritive base attained at pilot and industrial scale showed the following characteristics: loss due to desiccation, 1.93%; aminic nitrogen, 1.