A quick and reliable method for monitoring gene expression in actinomycetes.

Amycolatopsis balhimycina DSM5908 is the producer of the vancomycin-type glycopeptide antibiotic balhimycin. Balhimycin production is controlled by transcriptional regulators and depends on different environmental factors. To analyse the regulatory network P(tba), a representative promoter of the balhimycin gene cluster, was fused to the reporter gene egfp and integrated into the genome of A.