Infective Vaginal Discharge among Women of the Reproductive Age in the Outpatient Department of a Primary Care Centre.

Introduction: Infective vaginal discharge is the most common complaint of the women of reproductive age group attending gynaecology outpatient department. Vaginal discharge may be normal or abnormal. Infective vaginal discharge is usually related to one of the three conditions, like bacterial vaginosis, vulvovaginal candidiasis, and trichomoniasis.

Multidrug-resistant among Non-Fermenting Gram-negative Bacteria Isolated in the Department of Microbiology of a Tertiary Care Centre.

Introduction: Infection caused by Non-fermenting Gram-negative bacteria (NFGNB) like Pseudomonas aeruginosa and Acinetobacter baumannii leads to life-threatening conditions. These bacteria are often multidrug-resistant which leads to limited therapeutic options leading to treatment failure. Little information is available regarding the prevalence and resistance pattern of such bacteria in our country.

Multidrug Resistant Escherichia coli among Urinary Samples of Patients with Urinary Tract Infection in the Microbiology Department of a Tertiary Care Center: A Descriptive Cross-Sectional Study.

Introduction: Urinary Tract Infection one of the most common and manageable infections still holds its position as a major public health issue worldwide due to an increase in the number of multidrug resistant bacteria. This study aims to find out the prevalence of multidrug resistant Escherichia coli among urinary samples of patients with urinary tract infections in the microbiology Department of a tertiary care center.

Methods: A descriptive cross-sectional study was carried out at a tertiary care centre from 8 August 2018 to 9 January 2019.

Phenotypical Detection of β-Lactamases in a Multidrug-Resistant and Extensively Drug-Resistant : A Rare Human Pathogen With Special References to Risk Factor.

is gram-negative bacteria that cause infection in humans. It is less frequently isolated in the laboratory. The development of drug-resistant and its intrinsic ability to resist a wide range of antimicrobials enables them to cause mortality in an immunocompromised patient with a longer hospital stay.

Comparative genomic analyses of multi-drug resistant Mycobacterium tuberculosis from Nepal and other geographical locations.

Nepal exhibits a tuberculosis (TB) incidence rate that is comparable to neighbouring high TB incidence countries. In addition, it records >500 cases of multi-drug resistant (MDR) TB each year. The objective of this study was to perform whole-genome bioinformatic analysis on MDR-TB isolates from Nepal (n = 19) to identify the specific mutations underlying their phenotypic resistance.

Diagnostic performance of GeneXpert MTB/RIF assay compared to conventional culture for diagnosis of pulmonary and extrapulmonary tuberculosis, Nepal.

Tuberculosis is an infectious disease caused by the . It is a global health problem and major cause of death in resource-limited countries like Nepal. Timely diagnosis with sensitive testing methods could assist in early management of the disease.

Biofilm and metallo beta-lactamase production among the strains of Pseudomonas aeruginosa and Acinetobacter spp. at a Tertiary Care Hospital in Kathmandu, Nepal.

Introduction: Pseudomonas aeruginosa and Acinetobacter spp. are found to be associated with biofilm and metallo-β-lactamase production and are the common causes of serious infections mainly in hospitalized patients. So, the main aims of this study were to determine the rates of biofilm production and metallo beta-lactamase production (MBL) among the strains of Pseudomonas aeruginosa and Acinetobacter spp.

Detection of Methicillin Resistant and Determination of Minimum Inhibitory Concentration of Vancomycin for Isolated from Pus/Wound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal.

The present study was conducted to evaluate the performance of cefoxitin disc diffusion method and oxacillin broth microdilution method for detection of methicillin resistant (MRSA), taking presence of mecA gene as reference. In addition, inducible clindamycin resistance and beta-lactamase production were studied and minimum inhibitory concentration (MIC) of vancomycin for isolates was determined. A total of 711 nonrepeated pus/wound swab samples from different anatomic locations were included in the study.

Correlation between biofilm formation and resistance toward different commonly used antibiotics along with extended spectrum beta lactamase production in uropathogenic Escherichia coli isolated from the patients suspected of urinary tract infections visiting Shree Birendra Hospital, Chhauni, Kathmandu, Nepal.

Background: Escherichia coli is the most predominant causative agent of urinary tract infection (UTI). Recently, increase in drug resistance among the uropathogenic bacteria has caused great problem in treatment of UTI. The main objective of this research is to determine the correlation between biofilm formation and resistance toward different commonly used antibiotics along with extended spectrum beta lactamase production in uropathogenic Escherichia coli.