Cultivation of Limnospira maxima under extreme environmental conditions in Saudi Arabia: Salinity adaptation and scaling-up from laboratory culture to large-scale production.

Limnospira maxima has been adapted to grow in high salinity and in an economically alternative medium using industrial-grade fertilizers under harsh environmental conditions in Saudi Arabia. A sequence of scaling-up processes, from the laboratory to large-scale open raceways, was conducted along with gradual adaptation to environmental stress (salinity, light, temperature, pH). High biomass concentration at harvest point and areal productivity were achieved during the harsh summer season (1.

Cultivation of the polyextremophile 10D during summer conditions on the coast of the Red Sea and its adaptation to hypersaline sea water.

The west coast of the Arabian Peninsula borders the Red Sea, a water body which maintains high average temperatures and increased salinity compared to other seas or oceans. This geography has many resources which could be used to support algal biotechnology efforts in bio-resource circularity. However, summer conditions in this region may exceed the temperature tolerance of most currently cultivated microalgae.

Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site.

Sewer systems are complex physical, chemical and microbial ecosystems where fats, oils and grease (FOG) present a major problem for sewer management. Their accumulation can lead to blockages ('Fatbergs'), sewer overflows and disruption of downstream wastewater treatment. Further advancements of biological FOG treatments need to be tailored to degrade the FOG, and operate successfully within the sewer environment.

Enabling large-scale production of algal oil in continuous output mode.

Large-scale algal oil production requires continuous outputs and a trade-off between growth and oil content. Two unrelated marine algae ( [CCAP 849/10] and [CCAP 211/21A]) that showed high oil production under batch culture were studied under controlled semicontinuous cultivation conditions. Three essential attributes maximized oil productivity: (i) downregulation of cell size to maximize light absorption under N limitation; (ii) low nutrient-depletion thresholds to trigger oil induction; (iii) a means of carbohydrate suppression in favor of oil.

Co-culturing microbial consortia: approaches for applications in biomanufacturing and bioprocessing.

The application of microbial co-cultures is now recognized in the fields of biotechnology, ecology, and medicine. Understanding the biological interactions that govern the association of microorganisms would shape the way in which artificial/synthetic co-cultures or consortia are developed. The ability to accurately predict and control cell-to-cell interactions fully would be a significant enabler in synthetic biology.

Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices.

For the growing human population to be sustained during present climatic changes, enhanced quality and quantity of crops are essential to enable food security worldwide. The current consensus is that we need to make a transition from a petroleum-based to a bio-based economy via the development of a sustainable circular economy and biorefinery approaches. Both macroalgae (seaweeds) and microalgae have been long considered a rich source of plant biostimulants with an attractive business opportunity in agronomy and agro-industries.

Influence of nutrient status on the biohydrogen and lipid productivity in Parachlorella kessleri: a biorefinery approach.

The commercial reality of microalgal biotechnology for the production of individual bioactives is constrained by the high cost of production and requires a biorefinery approach. In this investigation, we examined the influence of different nutrient deprivation (nitrogen (N), phosphorus (P), sulphur (S) and manganese (Mn)) on growth, chlorophyll a (Chl a), biohydrogen (H) and fatty acid profiles in Parachlorella kessleri EMCCN 3073 under both aerobic and anaerobic conditions. Anaerobic conditions combined with the nutrient deprivation resulted in cell division blockage, reduction in Chl a and remarkable changes in pH, whereas a significant increase in the H production was observed after 24 h.

Metabolic Insights Into Infochemicals Induced Colony Formation and Flocculation in Unraveled by Quantitative Proteomics.

Microalgae can respond to natural cues from crustacean grazers, such as , by forming colonies and aggregations called flocs. Combining microalgal biology, physiological ecology, and quantitative proteomics, we identified how infochemicals from trigger physiological and cellular level changes in the microalga , underpinning colony formation and flocculation. We discovered that flocculation occurs at an energy-demanding 'alarm' phase, with an important role proposed in cysteine synthesis.

Phaeodactylum tricornutum: A Diatom Cell Factory.

A switch from a petroleum-based to a biobased economy requires the capacity to produce both high-value low-volume and low-value high-volume products. Recent evidence supports the development of microalgae-based microbial cell factories with the objective of establishing environmentally sustainable manufacturing solutions. Diatoms display rich diversity and potential in this regard.

Effects of cryopreservation on viability and functional stability of an industrially relevant alga.

As algal biotechnology develops, there is an increasing requirement to conserve cultures without the cost, time and genetic stability implications of conventional serial transfers, including issues regarding potential loss by failure to regrow, contamination on transfer, mix up and/or errors in the documentation on transfer. Furthermore, it is crucial to ensure both viability and functionality are retained by stored stock-cultures. Low temperature storage, ranging from the use of domestic freezers to storage under liquid nitrogen, is widely being used, but the implication to stability and function rarely investigated.

Quenching for Microalgal Metabolomics: A Case Study on the Unicellular Eukaryotic Green Alga .

Capturing a valid snapshot of the metabolome requires rapid quenching of enzyme activities. This is a crucial step in order to halt the constant flux of metabolism and high turnover rate of metabolites. Quenching with cold aqueous methanol is treated as a gold standard so far, however, reliability of metabolomics data obtained is in question due to potential problems connected to leakage of intracellular metabolites.

The Effect of High-Intensity Ultraviolet Light to Elicit Microalgal Cell Lysis and Enhance Lipid Extraction.

Currently, the energy required to produce biofuel from algae is 1.38 times the energy available from the fuel. Current methods do not deliver scalable, commercially viable cell wall disruption, which creates a bottleneck on downstream processing.

Effect of ammonium and high light intensity on the accumulation of lipids in (CCAP 849/10) and (CCAP 1055/1).

Background: Microalgae accumulate lipids when exposed to stressful conditions such as nutrient limitation that can be used to generate biofuels. Nitrogen limitation or deprivation is a strategy widely employed to elicit this response. However, this strategy is associated with a reduction in the microalgal growth, leading to overall poor lipid productivities.

Microwave-Assisted Extraction for Microalgae: From Biofuels to Biorefinery.

The commercial reality of bioactive compounds and oil production from microalgal species is constrained by the high cost of production. Downstream processing, which includes harvesting and extraction, can account for 70-80% of the total cost of production. Consequently, from an economic perspective extraction technologies need to be improved.

Harvesting Environmental Microalgal Blooms for Remediation and Resource Recovery: A Laboratory Scale Investigation with Economic and Microbial Community Impact Assessment.

A laboratory based microflotation rig termed efficient FLOtation of Algae Technology (eFLOAT) was used to optimise parameters for harvesting microalgal biomass from eutrophic water systems. This was performed for the dual objectives of remediation (nutrient removal) and resource recovery. Preliminary experiments demonstrated that chitosan was more efficient than alum for flocculation of biomass and the presence of bacteria could play a positive role and reduce flocculant application rates under the natural conditions tested.

Microbial consortia: a critical look at microalgae co-cultures for enhanced biomanufacturing.

Monocultures have been the preferred production route in the bio-industry, where contamination has been a major bottleneck. In nature, microorganisms usually exist as part of organized communities and consortia, gaining benefits from co-habitation, keeping invaders at bay. There is increasing interest in the use of co-cultures to tackle contamination issues, and simultaneously increase productivity and product diversity.

Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: a case study with the metastatic breast cancer cell line MDA-MB-231.

Metabolome characterisation is a powerful tool in oncology. To obtain a valid description of the intracellular metabolome, two of the preparatory steps are crucial, namely washing and quenching. Washing must effectively remove the extracellular media components and quenching should stop the metabolic activities within the cell, without altering the membrane integrity of the cell.

The Search for a Lipid Trigger: The Effect of Salt Stress on the Lipid Profile of the Model Microalgal Species for Biofuels Production.

Background: Algal cells produce neutral lipid when stressed and this can be used to generate biodiesel.

Objective: Salt stressed cells of the model microalgal species Chlamydomonas reinhardtii were tested for their suitability to produce lipid for biodiesel.

Methods: The starchless mutant of C.