Biochar facilitated Biological CO conversion to C2-C6 alcohols and fatty acids.

Microbial CO utilization reduces the carbon footprint, providing economic potential. Biochar, rich in minerals and trace metals, can enhance microbial activity. This study investigates poultry litter and switchgrass biochars produced at 350 and 700 °C (PLB350, PLB700, SGB350 and SGB700, respectively) affect CO conversion to C2-C6 alcohols and acids by Clostridium muellerianum P21, C.

Biochar amended microbial conversion of C1 gases to ethanol and butanol: Effects of biochar feedstock type and processing temperature.

Biochar feedstock and production method affects its physicochemical properties and subsequent application. This study investigated the effects of biochar from switchgrass (SGB) and poultry litter (PLB) produced at 350 and 700 °C on alcohol formation using CO:CO:H (40:30:30) by Clostridium carboxidivorans (P7) and C. ragsdalei (P11).

Improved Production and Characterization of Recombinant Human Granulocyte Colony Stimulating Factor from E. coli under Optimized Downstream Processes.

This work reports the upstream and downstream process of recombinant human granulocyte colony stimulating factor (rhG-CSF) expressed in Escherichia coli BL21 (DE3)pLysS. The fed batch mode was selected for the maximum output of biomass (6.4g/L) and purified rhG-CSF (136mg/L) under suitable physicochemical environment.

Simplified in vitro refolding and purification of recombinant human granulocyte colony stimulating factor using protein folding cation exchange chromatography.

Protein folding-strong cation exchange chromatography (PF-SCX) has been employed for efficient refolding with simultaneous purification of recombinant human granulocyte colony stimulating factor (rhG-CSF). To acquire a soluble form of renatured and purified rhG-CSF, various chromatographic conditions, including the mobile phase composition and pH was evaluated. Additionally, the effects of additives such as urea, amino acids, polyols, sugars, oxidizing agents and their amalgamations were also investigated.