Bulk Condensation by an Active Interface.

We present experiments, supported by mechanically detailed simulations, establishing bulk condensation of a hard-bead fluid by a tiny population of orientable motile grains that self-assembles into a moving polarized monolayer. In a quasi-1D geometry two such layers, oppositely aligned, immobilize the condensed nonmotile component. We account for our observations through a continuum theory with a naturally nonreciprocal Cahn-Hilliard structure, whose predicted trends as a function of packing fraction are consistent with our observations.

Polyhydroxyalkanoate Production by Methanotrophs: Recent Updates and Perspectives.

Methanotrophs are bacteria that consume methane (CH) as their sole carbon and energy source. These microorganisms play a crucial role in the carbon cycle by metabolizing CH (the greenhouse gas), into cellular biomass and carbon dioxide (CO). Polyhydroxyalkanoates (PHAs) are biopolymers produced by various microorganisms, including methanotrophs.

Novel cofactor regeneration-based magnetic metal-organic framework for cascade enzymatic conversion of biomass-derived bioethanol to acetoin.

Upgrading biomass-derived bioethanol to higher-order alcohols using conventional biotechnological approaches is challenging. Herein, a novel, magnetic metal-organic-framework-based cofactor regeneration system was developed using ethanol dehydrogenase (EtDH:D46G), NADH oxidase (NOX), formolase (FLS:L482S), and nicotinamide adenine dinucleotide (NAD) for converting rice straw-derived bioethanol to acetoin. A magnetic zeolitic imidazolate framework-8@FeO/NAD (ZIF-8@FeO/NAD) regeneration system for cell-free cascade reactions was introduced and used to encapsulate EtDH:D46G, NOX, and FLS:L482S (ENF).

Nonsterile Process for Biohydrogen Production: Recent Updates, Challenges, and Opportunities.

Hydrogen (H), a clean and versatile energy carrier, has recently gained significant attention as a potential solution for reducing carbon emissions and promoting sustainable energy systems. The yield and efficiency of the biological H production process primarily depend on sterilization conditions. Various strategies, such as heat inactivation and membrane-based sterilization, have been used to achieve desirable yields via microbial fermentation.

Laccase-Based Magnetic Inorganic-Protein Hybrid Nanobiocatalyst for Efficient Decolorization of Dyes in the Presence of Inhibitors.

In the present investigation, an ecofriendly magnetic inorganic-protein hybrid system-based enzyme immobilization was developed using partially purified laccase from (Lac), FeO nanoparticles, and manganese (Mn), and was successfully applied for synthetic dye decolorization in the presence of enzyme inhibitors. After the partial purification of crude Lac, the specific enzyme activity reached 212 U∙mg total protein. The synthesized FeO/Mn(PO)-laccase (FeO/Mn-Lac) and Mn(PO)-laccase (Mn-Lac) nanoflowers (NFs) exhibited encapsulation yields of 85.

Sequential Co-Immobilization of Enzymes on Magnetic Nanoparticles for Efficient l-Xylulose Production.

Multi-enzymatic strategies have shown improvement in bioconversion during cofactor regeneration. In this study, purified l-arabinitol 4-dehydrogenase (LAD) and nicotinamide adenine dinucleotide oxidase (Nox) were immobilized via individual, mixed, and sequential co-immobilization approaches on magnetic nanoparticles, and were evaluated to enhance the conversion of l-arabinitol to l-xylulose. Initially, the immobilization of LAD or Nox on the nanoparticles resulted in a maximum immobilization yield and relative activity of 91.

Sarcoidosis associated with Trastuzumab in a young female with carcinoma of breast.

Trastuzumab is a humanized monoclonal antibody against human epidermal growth factor, useful in breast cancer. It is well tolerated but sometimes may associate with significant adverse effects. Sarcoidosis is a granulomatous disease involving multiple organs with poorly understood aetiology.

Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas.

The production of renewable energy or biochemicals is gaining more attention to minimize the emissions of greenhouse gases such as methane (CH) and carbon dioxide for sustainable development. In the present study, the influence of copper (Cu)- and iron (Fe)-based nanoparticles (NPs), such as Cu, FeO, and CuFeO, was evaluated during the growth of methanotrophs for inoculum preparation and on the development of a polymeric-matrix-based encapsulation system to enhance methanol production from simulated biogas (CH and CO). The use of simulated biogas feed and the presence of NP-derived inoculums produce a remarkable enhancement in methanol production up to 149% and 167% for and free-cells-based bioconversion, respectively, compared with the use of pure CH as a control feed during the growth stage.

Coriolus versicolor laccase-based inorganic protein hybrid synthesis for application in biomass saccharification to enhance biological production of hydrogen and ethanol.

In this study, a bio-friendly inorganic protein hybrid-based enzyme immobilization system using partially purified Coriolus versicolor laccase (CvLac) was successfully applied to biomass hydrolysis for the enhancement of sugar production aimed at generating biofuels. After four days of incubation, the maximum CvLac production was achieved at 140 U/mg of total protein in the presence of inducers such as copper and wheat bran after four days of incubation. Crude CvLac immobilized through inorganic protein hybrids such as nanoflowers (NFs) using zinc as Zn(PO)/CvLac hybrid NFs (Zn/CvLac-NFs) showed a maximum encapsulation yield of 93.

Multifactorial role of nanoparticles in alleviating environmental stresses for sustainable crop production and protection.

In the era of dire environmental fluctuations, plants undergo several stressors during their life span, which severely impact their development and overall growth in negative aspects. Abiotic stress factors, especially moisture stress i.e shortage (drought) or excess (flooding), salinity, temperature divergence (i.

Initial experience with 3D laparoscopic choledochal cyst (CDC) excision and hepatico-duodenostomy (HD) in 21 children.

Introduction: Minimal access surgery has gradually become the standard of care in the management of choledochal cysts (CDC). Laparoscopic management of CDC is a technically challenging procedure that requires advanced intracorporeal suturing skills, and hence, has a steep learning curve. Robotic surgery has the advantages of 3D vision, articulating hand instruments making suturing easy and thus is ideal.

Morphometric delineation of administrative boundaries and classification of threatened categories of small watersheds in transboundary rivers.

The ecological conservation of large rivers is impossible unless immediate attention is given to protecting their small tributaries at local levels. The natural boundaries of large river basins are shrinking because their tributaries and streams of different orders are disappearing at an unprecedented rate. Delineation of the fixed administrative boundaries (AB) to protect the natural boundary of small rivers and their classification into appropriate threatened categories, the present study was carried out on the 54.

Role of rotational inertia for collective phenomena in active matter.

We investigate the effect of rotational inertia on the collective phenomena of underdamped active systems and show that the increase of the moment of inertia of each particle favors non-equilibrium phase coexistence, known as motility induced phase separation, and counteracts its suppression due to translational inertia. Our conclusion is supported by a non-equilibrium phase diagram (in the plane spanned by rotational inertial time and translational inertial time) whose transition line is understood theoretically through scaling arguments. In addition, rotational inertia increases the correlation length of the spatial velocity correlations in the dense cluster.

Synthetic design of methanotroph co-cultures and their immobilization within polymers containing magnetic nanoparticles to enhance methanol production from wheat straw-based biogas.

In this study, various methanotroph co-cultures were designed to enhance methanol production from biogas produced through the anaerobic digestion of wheat straw (WS). Furthermore, whole-cell immobilization was performed using magnetic nanoparticle (MNP)-loaded polymers to develop an efficient bioprocess. The anaerobic digestion of WS by cattle dung yielded 219 L/kg of total solids reduced.

Synergism of endophytic and modulates plant growth and bacoside biosynthesis in .

is the main source of pharmaceutically important bacosides; however, the low content of these molecules remains a limiting factor for fulfilling the industrial requirement. The accumulation of secondary metabolites can be enhanced in plants upon inoculation with endophytes. In this study, we isolated and analyzed the culturable endophytes associated with different plant parts.

An Unusual Case of Type A Posterior Cloaca Associated with 46XX Disorder of Sexual Differentiation with Y Duplication of Urethra.

We report an infant reared as a girl who presented with ambiguous genitalia and urine coming per rectum. On examination, she had minimal clitoromegaly with labial fusion and Y urethral duplication. On investigations, genetic, metabolic, and endocrine causes for disorder of sexual differentiation were ruled out.

Active nonreciprocal attraction between motile particles in an elastic medium.

We show from experiments and simulations on vibration-activated granular matter that self-propelled polar rods in an elastic medium on a substrate turn and move towards each other. We account for this effective attraction through a coarse-grained theory of a motile particle as a moving point-force density that creates elastic strains in the medium that reorient other particles. Our measurements confirm qualitatively the predicted features of the distortions created by the rods, including the |x|^{-1/2} tail of the trailing displacement field and nonreciprocal sensing and pursuit.

Recent Strategies for the Immobilization of Therapeutic Enzymes.

Therapeutic enzymes play important roles in modern medicine due to their high affinity and specificity. However, it is very expensive to use them in clinical medicine because of their low stability and bioavailability. To improve the stability and effectiveness of therapeutic enzymes, immobilization techniques have been employed to enhance the applications of therapeutic enzymes in the past few years.

Exploring health coaching and mindfulness as levers for transformation in health: stakeholder perspectives.

Background: Health coaching (HC) and mindfulness (MFN) are proven interventions for mobilizing patients' inner resources and are slowly being integrated into public primary care. Since 2015 the medical community in Gibsons BC has integrated physician-led HC and MFN-based programs into team-based care. This exploratory study aimed to understand the mechanisms by which these rural programs helped both patients and clinicians, and to elicit priorities for future study in these fields.

Correction to: Biomethanol Production from Methane by Immobilized Co-cultures of Methanotrophs.

[This corrects the article DOI: 10.1007/s12088-020-00883-6.].

Laccase Immobilization on Magnetic Nanoparticles to Improve Stability and Its Potential Application in Bisphenol A Degradation.

In the present study, laccase (Lac) was immobilized through covalent methods on the magnetic nanoparticles. FeO and FeO nanoparticles activated by 3-aminopropyltriethoxysilane followed with glutaraldehyde showed maximum immobilization yields and relative activity up to 81.4 and 84.

Integrating anaerobic digestion of potato peels to methanol production by methanotrophs immobilized on banana leaves.

In the present study, potato peels were subjected to anaerobic digestion (AD) to produce biogas (methane [CH] and carbon dioxide), which was subsequently used as a substrate for methanol production by methanotrophs. AD resulted in high yields of up to 170 L CH/kg total solids (TS) from 250 mL substrate (2% TS, w/v). Under optimized conditions, maximum methanol production of 4.

Multidisciplinary Approach for Management of Pediatric Tumors: a Case Series.

Pediatric solid tumors have wide range of presentations. Multidisciplinary approach is often needed for their optimum management. There are no reports discussing such an approach to pediatric tumors involving pediatric surgeons, oncologists and cardiothoracic surgeons together for the management.