Low vitamin D status throughout life results in an inflammatory prone status but does not alter bone mineral or strength in healthy 3-month-old CD-1 male mice.

Scope: The aim of this study was to assess if exposure to different levels of dietary vitamin D pre- and postweaning impacts the intestinal-bone axis.

Methods And Results: Female CD1 mice were exposed to high (5000 IU vitamin D3 /kg diet, H) or low (25 IU vitamin D3 /kg diet, L) vitamin D diet (modified AIN-93G) during pregnancy and lactation. At weaning (postnatal day 21), a subset of the male offspring was sacrificed and another subset was assigned to receive their dams' respective diet (HH and LL) or the other diet (HL and LH) until sacrifice at 3 months of age.

Impact of Bifidobacterium bifidum MIMBb75 on mouse intestinal microorganisms.

Bifidobacterium bifidum MIMBb75 is a recently identified probiotic. However, its distribution along the intestine and impact on resident microbiota is unknown. Herein, we established a quantitative real-time polymerase chain reaction assay targeting the B.