Publications by authors named "Raghavendra Amachawadi"

Liver abscesses, which occur in finishing cattle, are of significant economic concern to the feedlot industry. The causative agents include both subspecies ( and . ), (), and serotype Lubbock (S.

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Article Synopsis
  • - The study investigated Staphylococcus aureus in milk, finding a 34.4% prevalence, with 27% identified as methicillin-resistant (MRSA) and 24.71% showing multiple antimicrobial resistance (MAR) patterns.
  • - The isolates tested positive for enterotoxin genes sea and sed, and exhibited significant biofilm formation, indicating a potential health risk associated with contaminated milk.
  • - The research highlights the diverse genetic profiles of S. aureus and calls for ongoing monitoring and strategies to enhance food safety in Chennai, given its implications for public health.
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Introduction: Antimicrobial resistance (AMR) in , the causative agent of brucellosis, is of growing concern, particularly in low and middle-income countries. This study aimed to explore the genetic basis of AMR in strains from India.

Methods: Twenty-four isolates from humans and animals were subjected to antimicrobial susceptibility testing and whole-genome sequencing.

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We present the draft genome sequences of 23 isolates derived from human and animal sources across India with genome size predominantly at 3.207 M and uniform GC content (57.24%) across isolates.

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Diabetes mellitus (DM) is a common comorbidity in COVID-19 subjects. Hyperglycemia at hospital admission identified as a major risk factor and is responsible for poor prognosis. Hematological and inflammatory parameters have been recognized as predictive markers of severity in COVID-19.

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We isolated and characterized two lytic bacteriophages against named TANUVAS_MVC-VPHSA1 and TANUVAS_MVC-VPHSA2, with the aim of investigating their genomic and structural features. The bacteriophages belong to the Caudoviricetes, and their genomes have sizes of 50,505 and 50,516 base pairs with a GC content of 41.4%.

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Holstein steers (n = 40; initial BW = 84.9 ± 7.1 kg) were used to study the genesis of liver abscesses (LA) using an acidotic diet challenge with or without intraruminal bacterial inoculation.

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Bovine respiratory disease (BRD) is an economically important disease in feedyards influencing both animal welfare and antimicrobial utilization. Major pathogens associated with BRD have been identified in previous research, but little information is available on the relationship between nasopharyngeal microbiota and health outcomes. The objective of this study was to identify potential associations between nasopharyngeal microbiota and antimicrobial resistance patterns of clinical cases that lived or died compared to non-diseased controls.

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Antimicrobial resistance (AMR) in bacteria is a major public health concern in the US and around the world. is an important foodborne pathogen that resides in the gut of pigs and is shed in feces, with the potential to be transmitted to humans. In pigs, the oral route, either in-feed or in-water, is by far the most common route of administration of antimicrobials.

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The present work elucidates the fabrication of Barium Lanthanum Oxide nanosheets (BaLaO NSs) via a simple one-pot precipitation method. The acquired results show an orthorhombic crystal system with an average crystallite size of 27 nm. The morphological studies revealed irregular-shaped sheets stacked together in a layered structure, with the confirmation of the precursor elements.

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Article Synopsis
  • Milk is a nutrient-rich food that can contain harmful microorganisms, particularly a dangerous foodborne pathogen linked to infections and antimicrobial resistance (AMR) in animals.
  • A study in Chennai, India, analyzed 259 raw milk samples from dairy farms, local vendors, and retail outlets, finding 34% positive for the pathogen, with significant genetic diversity among the isolates.
  • The research noted that all isolates were resistant to ampicillin and identified 28 multidrug-resistant isolates, highlighting worries about food safety and the economic impact of AMR in milk sources.
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A total of 34,749 pigs were used in two experiments to evaluate the effects of a postbiotic dried fermentation product () administered through drinking water on nursery pig growth performance, antibiotic injection frequency, morbidity, mortality, fecal consistency, and characterization of fecal . The DFP is composed of bioactive molecules derived from In Exp. 1, 350 barrows (DNA Line 200 × 400; initial body weight [] 6.

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  • The study involved regular monitoring of body weights and blood samples over a period, with steers harvested at two different time points (3 and 10 days) for detailed examination of various organs for signs of disease.
  • Results showed no significant differences in overall health or liver abscess development between treatments, indicating that the LPS challenge combined with specific bacterial inoculations did not effectively induce liver abscesses in the steers within the study timeframe.
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Antimicrobials and heavy metals are commonly used in the animal feed industry. The role of in-feed antimicrobials on the evolution and persistence of resistance in enteric bacteria is not well described. Whole-Genome Sequencing (WGS) is widely used for genetic characterizations of bacterial isolates, including antimicrobial resistance, heavy metal tolerance, virulence factors, and relatedness to other sequenced isolates.

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Zinc oxide (ZnO) nanostructures, both undoped and Co-doped, were synthesized through the solution combustion process. The diffraction patterns from powder XRD revealed that the materials were crystalline. The morphology of the spherically formed nanoparticles was visualized in SEM micrographs.

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There is a great demand to replace non-renewable materials with eco-friendly renewable materials for many applications in recent times. In the present study, such an attempt was made to substitute synthetic polymer-based films used for food packaging applications with films prepared out of renewable materials derived from waste. The pectin/polyvinyl alcohol (PP) and pectin-MgO/polyvinyl alcohol (PMP) films were prepared and characterized to ascertain their suitability for packaging applications.

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Here, we report the genome sequence of a Pasteurella multocida strain isolated from the heart blood of a spotted deer (Bareilly, India). The 2.44-Mbp genome has 2,227 coding sequences, with a G+C content of 40.

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A total of 90 pigs, approximately one day of age, were used in a 42-day study to evaluate whether Endovac-Porci, a core antigen vaccine with an immunostimulant, provides piglets with broad-spectrum protection against the enteric and respiratory effects of Gram-negative bacteria. This study was a single-site, randomized, prospective, blinded, comparative placebo-controlled design. Individual pigs were randomly allocated to 1 of 2 treatments in a randomized design.

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In the recent survey, Japanese encephalitis (JE) is one of the most common mosquito-borne diseases, accounting for ∼30% of fatalities. The outbreaks of the JE virus (JEV) suggests that exhaustive study is essential for the prevention and management of the disease. The disease mainly spreads in humans and pigs by the vector: mosquito; as this is a major concern, this study had employed various bioinformatics tools to investigate the codon usage bias, evolutionary inference and selection pressure analysis of the Japanese encephalitis virus disease.

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Bacterial infections are one of the leading causes of morbidity, mortality, and healthcare complications in patients. Leptospirosis is found to be the most prevalent, re-emergent, and neglected tropical zoonotic disease worldwide. The adaptation to various environmental conditions has made acquire a large genome (~4.

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Probiotic microbiota plays a vital role in gastrointestinal health and possesses other beneficial attributes such as antimicrobial and antibiotic agents along with a significant role in the management of diabetes. The present study identifies the probiotic potential of spp. isolated from three traditionally fermented foods namely, jalebi, medhu vada, and kallappam batters at biochemical, physiological, and molecular levels.

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Liver abscesses in feedlot cattle are a polymicrobial infection with Fusobacterium necrophorum and Trueperella pyogenes as the primary and secondary etiologic agents, respectively. Cattle with liver abscesses do not exhibit clinical signs and the abscesses are detected only at slaughter. The objective was to conduct metabolomics analysis of purulent materials of liver abscesses to identify biochemicals.

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is a spore-forming, anaerobic bacterium which produces toxins and exoenzymes that cause disease in calves, especially necro-hemorrhagic enteritis-associated diarrhea often resulting in death. infections are currently being treated with antibiotics, but even with the prudent administration of antibiotics, there are significant rates of recurrence. Probiotics, an alternative to antibiotics, are commonly employed to prevent clostridial infections.

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Agricultural residues are suitable to make useful products that can potentially replace the non-biodegradable polymeric materials. In the present work, corn cob and rice S=straw is quantitatively transformed to cellulose by alkali treatment preceded by bleaching. The obtained cellulose is changed into nanocellulose (NC) by the acid hydrolysis method followed by ultrasonication.

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Salmonella enterica, which causes typhoid fever, is one of the most prevalent food-borne pathogens. Salmonellosis in cattle can greatly impact a producer’s income due to treatment costs, decreased productivity of the herd, and mortality due to disease. Current methods of treatment and prevention for salmonellosis consist of antibiotics and vaccinations, but neither of these options are perfect.

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