This study investigates the in vitro digestion of vacuum-impregnated snacks enriched with and mango seed polyphenols, focusing on bacterial survival and polyphenol bioaccessibility. The snacks were prepared by vacuum impregnation (VI) with solutions containing either mango seed extract, , or a combination of both, followed by dehydration. The antimicrobial activity of the treatments was assessed against pathogens, revealing limited effectiveness, likely due to insufficient concentrations of mango seed extract and the intrinsic resistance of the bacteria.
View Article and Find Full Text PDFJackfruit leaf protein hydrolysates obtained from the enzymatic hydrolysis of leaf protein concentrate with gastrointestinal enzymes have shown good techno-functional properties and high antioxidant capacity. However, molecular weight, antiproliferative activity, cytotoxicity and the ability to reduce reactive oxygen species (ROS) are still unknown. Therefore, this study aimed to evaluate the effect of jackfruit leaf protein hydrolysates obtained by enzymatic hydrolysis with pepsin and pancreatin at different hydrolysis times (30-240 min) on molecular weights, cytotoxicity, antiproliferation of cancer cells, and the reduction of reactive oxygen species in HO-induced HaCaT cells.
View Article and Find Full Text PDFMexican Coccoloba uvifera fruit contains polyphenols, flavonoids, and anthocyanins, while in the leaves, lupeol, α- and β-amyrin have been previously identified by HPLC. However, the low resolution by HPLC of pentacyclic triterpenes (PTs) is a limitation. Moreover, the volatile profile of C.
View Article and Find Full Text PDFUnlabelled: The combined impact of high-hydrostatic pressure (HHP) and ultrasound (US) on the cyanidin-3-O-rutinoside (C3R), quercetin-3-O-rutinoside (Q3R), and volatile compounds from fig () paste was investigated. The HHP increased the content of C3R and Q3R, from 70 to 133 mg/kg fw and 31 to 44 mg/kg fw, respectively. The combination of HHP and US further enhanced the extraction of these bioactive compounds.
View Article and Find Full Text PDFBackground: Anthracnose caused by species of Colletotrichum is the most important disease of avocado fruit. The quiescent infection develops in the field, hence, its control from the preharvest stage is necessary. The field application of microencapsulated Yamadazyma mexicana LPa14 could prevent the establishment of Colletotrichum gloeosporioides and reduce the losses in avocado production.
View Article and Find Full Text PDFYam bean is an important source of dietary fiber and other components that comprise the total indigestible fraction (TIF), which can be fermented by the colonic microbiota and produce metabolites with beneficial health effects. Therefore, the objective of this study was to evaluate the in vitro colonic fermentation of yam bean TIF and the changes caused by the addition of a polyphenolic extract of mango seed and the lactic acid bacteria Pediococcus acidilactici. The mango seed extract was obtained by ultrasound-assisted extraction, and the microbial growth rate and viability of P.
View Article and Find Full Text PDFBiology (Basel)
May 2023
Since the fruits of and are rich in proteases, the aim of this research was to optimize the hydrolysis process of cooked white shrimp by-products due to the effect of these proteases. A robust Taguchi L design was used to optimize the hydrolysis process. Similarly, the amino acid profile by GC-MS and antioxidant capacity (ABTS and FRAP) were determined.
View Article and Find Full Text PDFHigh hydrostatic pressure (HHP) is a non-thermal process widely used in the food industry to reduce microbial populations. However, rarely its effect has been assessed in products with high oil content. This study evaluated the efficacy of HHP (200, 250, and 300 MPa) at different temperatures (25, 35, and 45 °C) by cycles (1, 2, or 3) of 10 min in the inactivation of Aspergillus niger spores in a lipid emulsion.
View Article and Find Full Text PDFJ Microbiol Methods
January 2023
A new standardized method, using isothermal microcalorimetry (IMC), was established to determine thermokinetic parameters from heat flow curves and to demonstrate the reproducibility and repeatability of the parameters of five Colletotrichum species on different days. Measurements on IMC were made at different periods and by two operators. Repeatability and reproducibility (R&R) measurement system analysis was performed on the technique used to measure the heat flow of Colletotrichum strains.
View Article and Find Full Text PDFThis study aimed to microencapsulate the sea grape ethanolic extract by the spray drying process, characterizing the obtained powder, and evaluating its antimutagenicity activity. Microparticles showed a mean size of 6.28 μm and a spherical shape with a smooth surface.
View Article and Find Full Text PDFJ Microbiol Methods
April 2022
Conventional microbiological methods to evaluate the in vitro antifungal activity of bioactive compounds usually consume a long time. It is also difficult to calculate different kinetic parameters. For this reason, this study aimed to evaluate the sensitivity of phytopathogenic fungi to an ethanolic extract of jackfruit leaf by the poison agar and isothermal microcalorimetry (IMC) tests.
View Article and Find Full Text PDFBackground: The Coccoloba uvifera L. species is currently considered an important source of compounds of high biological value such as lupeol. This is related to different and important biological activities to human health.
View Article and Find Full Text PDFThe electrospinnability of FucoPol, a bacterial exopolysaccharide, is presented for the first time, evaluated alone and in combination with other polymers, such as polyethylene oxide (PEO) and pullulan. The obtained fibers were characterized in terms of their morphological, structural and thermal properties. Pure FucoPol fibers could not be obtained due to FucoPol's low water solubility and a lack of molecular entanglements.
View Article and Find Full Text PDFThe extracts are receiving attention due to their agro-food applications. Then, the simultaneous optimization of microwave-assisted extraction of polyphenols from jackfruit leaf with growth inhibitory action against sp. was studied.
View Article and Find Full Text PDFJackfruit ( Lam.) is an evergreen tree that produces a high waste of leaves. This study evaluated the obtention of peptides from jackfruit leaves using pancreatin and pepsin, their antifungal activity, and their effect on pectin films.
View Article and Find Full Text PDFThis study aimed to evaluate the encapsulating potential of a jackfruit leaf protein hydrolysate, through obtaining pentacyclic triterpenes-rich extract loaded nanoemulsion. Response surface methodology (RSM) was used to optimize the conditions to obtain an optimal nanoemulsion (NE-Opt). The effect of protein hydrolysate concentration (0.
View Article and Find Full Text PDFGlobal demand for minimally processed fruits and vegetables is increasing due to the tendency to acquire a healthy lifestyle. Losses of these foods during the chain supply reach as much as 30%; reducing them represents a challenge for the industry and scientific sectors. The use of edible packaging based on biopolymers is an alternative to mitigate the negative impact of conventional films and coatings on environmental and human health.
View Article and Find Full Text PDFUnlabelled: The aim of this work was to evaluate the technologies effect of cold extraction by centrifugation (CE) and ultrasound-assisted (US-CE) methods without adding water, on the avocado oil yield, nutritional composition, physicochemical characteristics, oxidative stability (oxidation temperature and time, besides activation energy) and accelerated shelf life regarding hexane extraction (control). The US-CE improved the physicochemical properties such as acidity, peroxides, and iodine indexes regarding CE and Control. US-CE improved the yield, nutritional quality of fatty acids, oxidative stability, shelf life, and ω-6/ω-3 ratio regarding CE.
View Article and Find Full Text PDFJ Sci Food Agric
January 2022
Background: Microorganism for biological control of fruit diseases is an eco-friendly alternative to the use of chemical fungicides.
Results: This is the first study evaluating the electrospraying process to encapsulate the biocontrol yeast Meyerozyma caribbica. The effect of encapsulating material [Wey protein concentrate (WPC), Fibersol® and Trehalose], its concentration and storage temperature on the cell viability of M.
This study focuses on the production and characterization of electrosprayed cashew gum (CG) microparticles that encapsulate β-carotene. CG is an inexpensive, non-toxic polysaccharide obtained from Anacardium occidentale trees. Encapsulation of β-carotene in CG was performed by electrospraying from two emulsion formulations (water : oil ratios 80:20 and 90:10 (v/v)) in which the dispersed phase consisted of β-carotene dissolved in castor oil, and the continuous phase was a CG aqueous solution.
View Article and Find Full Text PDFIn this work, different whey protein (WP) ratios (5, 10, 20, 30, 40 and 50% w/w) were added as stabilizers to high degree of polymerization Agave fructans (HDPAF) capsules to decrease the hygroscopicity. Results showed that the WP and HDPAF in 1:520:80 ratio (20/80 w/w) decreased significantly the hygroscopicity of capsules from 12.19 to 8.
View Article and Find Full Text PDFThe volatile compounds of five kind of cultivars of jackfruit (Artocarpus heterophyllus Lam.) grown in Nayarit, Mexico, was researched by using extraction and chromatographic methods such as headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). Eighty-six volatile compounds were identified.
View Article and Find Full Text PDFBiotechnol Appl Biochem
February 2022
The objective of this work was to evaluate the biochemical characteristics of an enzymatic extract obtained from autochthonous fungus Aspergillus niger ITV02 and its application in the enzymatic hydrolysis of wheat straw and corn stubble pretreated by steam explosion. The enzymatic extract was obtained by submerged fermentation using delignified sweet sorghum bagasse as a carbon source. The results obtained showed that the enzymatic extract had β-glucosidase and endoglucanase activities.
View Article and Find Full Text PDFBackground: Cancer is a disease characterized by the invasion and uncontrolled growth of cells. One of the best ways to minimize the harmful effects of mutagens is through the use of natural antimutagens. In this regard, the search for new antimutagens that act in the chemoprevention could represent a promising field in this area.
View Article and Find Full Text PDFThe application of emerging technologies such as ultrasound, microwaves and high hydrostatic pressure, allows the extraction of compounds in a sustainable manner from a vegetable matrix with a high value such as jackfruit leaf proteins ( Lam). Currently, the main method of protein extraction is based on the precipitation with the use of an aggressive solvent, therefore it is necessary to optimize extraction methods with a minimum waste production. In the protein extraction of jackfruit leaves, we obtained a content of 84.
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