Publications by authors named "Rafik Hamed Sayed"
Article Synopsis
- Infectious coryza (IC) is a serious respiratory disease in chickens caused by Avibacterium paragallinarum, leading to higher culling in meat chickens and significant drops in egg production for laying hens.
- The study investigates the use of various nanoparticles (NPs) like silicon dioxide and iron oxide in developing new vaccines and examining their antibacterial effects on the bacteria causing IC.
- Results indicate that a concentration of 400 µg/ml of NPs effectively inactivates A. paragallinarum, with the SiO NP-adjuvanted vaccine generating the strongest immune response compared to other nanoparticle formulations and a commercial vaccine.
Objective: This work was conducted for the development of a 5-combi lateral flow immunochromatographic kit (LFK) for rapid and simultaneous identification of the common bacterial causes of bovine mastitis. The following pathogens are the identification targets of this kit: , , , , and in milk samples from suspected bovine mastitis cases. The conventional microbiological identification of these agents is not only time-consuming and requires a fully equipped laboratory but also requires experienced personnel.
View Article and Find Full Text PDFDermatophytosis is a widely spread contagious zoonotic disease, affecting both man (tinea) and animals (ringworm). This disease is caused by a group of closely related keratinophilic fungi known collectively as the dermatophytes group. Although the wide distribution of dermatophytosis cases throughout the whole world and its adverse clinical effect on human health, economical effect on productive animals, and pet animal welfare, there is no rapid accurate diagnostic tool for such disease.
View Article and Find Full Text PDFBackground And Aim: Brucellosis is a zoonotic disease with a worldwide distribution. It has a serious impact on the health of humans and animals, along with a negative impact on the economy. This study aimed to prepare and evaluate the diagnostic performance of a lateral flow immunochromatographic test (LFIT) nanogold diagnostic kit for detecting brucellosis in sheep.
View Article and Find Full Text PDFIn the presented study, we developed a nanogold lateral glow immunoassay-based technique (LFI-COVID-19 antigen test) for the detection of SARS-CoV-2 nucleocapsid proteins; the developed LFI-COVID-19 Ag test has been tested for limit of detection (LOD), cross-reactivity and interfering substances, and performance. It was found that the performance of the developed LFI-COVID-19 antigen test when it was evaluated by RT-qPCR indicated 95, 98, and 97% for sensitivity, specificity and accuracy, respectively. This complies with the WHO guidelines.
View Article and Find Full Text PDFAim: The present work aimed to develop lateral flow immunochromatographic strip (ICS) test for detection of Enteritidis (SE) specific antibodies in chicken sera.
Materials And Methods: A rapid lateral flow immunochromatographic test (LFIT) has been developed, in which SE Group D antigen labeled with the gold chloride molecules laid on the conjugate pad. protein A was used as capture antibody at the test line (T) of a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit antibodies were used as capture antibody at the control line (C) of the NC strip in the lateral flow layout device.