The main criteria for mate selection: The nursing students' perspective in the south-west of Iran during 2020-2021: A cross-sectional study.

Background And Aims: Marriage has been a functional and moral relationship between men and women, which leads to the creation of a family and also can result in the birth of a child. This study aimed at determining the mate-selection criteria of nursing students.

Methods: 298 nursing students were considered in this cross-sectional, descriptive-analytical study.

Enriched microbial communities for ammonium and nitrite removal from recirculating aquaculture systems.

The aim of this study was the enrichment of high-performance microbial communities in biofilters for removal of ammonium and nitrite from aquaculture water. Ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were enriched from different environmental water samples. The microbial communities with higher ammonium and nitrite removal activity were selected and adapted to different temperatures [9 °C, 15 °C, room temperature (25 °C), and 30 °C].

The effect of photodynamic therapy by gold nanoparticles on Streptococcus mutans and biofilm formation: an in vitro study.

In this experimental study, we aimed to evaluate the antibacterial and anti-biofilm effects of photodynamic therapy with a photosensitizer in conjunction with Gold nanoparticles against Streptococcus mutans as an important cariogenic bacterial agent. This experimental in vitro study evaluated the antibacterial and anti-biofilm effect of five groups as followed against S. mutans: methylene blue (MB), Gold nanoparticles (AuNPs), methylene blue conjugated with Gold nanoparticles (MB-AuNPs), MB mediated photodynamic therapy (MB mediated PDT) and methylene blue conjugated with Gold nanoparticles mediated photodynamic therapy (MB-AuNPs mediated PDT).

Abatement of water nutrient load in a fish culture system using the aquatic trophic levels.

Integrated aquatic systems are used to decrease the nutrient loads of effluents negating the negative environmental impacts of aquacultural systems. Some of these systems have a separate algae compartment requiring high maintenance. An integrated culture system was set up with different trophic levels: algae, zooplankton, and fish.

Toxicity of polystyrene microplastics on juvenile Oncorhynchus mykiss (rainbow trout) after individual and combined exposure with chlorpyrifos‎.

Microplastic (MP) sorption and transfer of chemical contaminants has been widely reported, yet few studies have investigated combined effects of contaminant-loaded MPs on organisms. This study examined effects of pristine or chlorpyrifos (CPF)-loaded polystyrene (PS)‎ fragments on histopathological and histomorphometrical biomarkers in rainbow trout (Onchorhynchus mykiss)‎. In laboratory, O.

Time, pattern, and outcome of medulloblastoma relapse and their association with tumour biology at diagnosis and therapy: a multicentre cohort study.

Background: Disease relapse occurs in around 30% of children with medulloblastoma, and is almost universally fatal. We aimed to establish whether the clinical and molecular characteristics of the disease at diagnosis are associated with the nature of relapse and subsequent disease course, and whether these associations could inform clinical management.

Methods: In this multicentre cohort study we comprehensively surveyed the clinical features of medulloblastoma relapse (time to relapse, pattern of relapse, time from relapse to death, and overall outcome) in centrally reviewed patients who relapsed following standard upfront therapies, from 16 UK Children's Cancer and Leukaemia Group institutions and four collaborating centres.

The molecular landscape and associated clinical experience in infant medulloblastoma: prognostic significance of second-generation subtypes.

Aims: Biomarker-driven therapies have not been developed for infant medulloblastoma (iMB). We sought to robustly sub-classify iMB, and proffer strategies for personalized, risk-adapted therapies.

Methods: We characterized the iMB molecular landscape, including second-generation subtyping, and the associated retrospective clinical experience, using large independent discovery/validation cohorts (n = 387).

Efficient Noisy Sound-Event Mixture Classification Using Adaptive-Sparse Complex-Valued Matrix Factorization and OvsO SVM.

This paper proposes a solution for events classification from a sole noisy mixture that consist of two major steps: a sound-event separation and a sound-event classification. The traditional complex nonnegative matrix factorization (CMF) is extended by cooperation with the optimal adaptive sparsity to decompose a noisy single-channel mixture. The proposed adaptive sparsity CMF algorithm encodes the spectra pattern and estimates the phase of the original signals in time-frequency representation.

The growth performance, antioxidant capacity, immunological responses, and the resistance against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus) fed Pistacia vera hulls derived polysaccharide.

The present study aimed at evaluating the supplementation of dietary Pistacia vera hulls derived polysaccharide (PHDP) at varying levels (0, 2.5, 5, and 10 g/kg diet) on the growth rate, digestive enzyme activity, immune response, and antioxidative capacity of Nile tilapia. After the feeding trial (60 days), fish were injected intraperitoneally with Aeromonas hydrophila, and the cumulative mortality was calculated for 10 days.

Cold-Resistant Heterotrophic Ammonium and Nitrite-Removing Bacteria Improve Aquaculture Conditions of Rainbow Trout (Oncorhynchus mykiss).

The aim of this study was isolation and characterization of heterotrophic bacteria capable of ammonium and nitrite removal at 15 °C (optimal temperature for growing rainbow trout Oncorhynchus mykiss). Environmental isolates were grown in liquid media containing ammonium or nitrite, and best strains in terms of growth and ammonium or nitrite removal were identified via 16S rRNA sequencing. Dyadobacter sp.

Effects of dietary Lactobacillus plantarum (KC426951) in biofloc and stagnant-renewal culture systems on growth performance, mucosal parameters, and serum innate responses of Nile tilapia Oreochromis niloticus.

Effects of dietary Lactobacillus plantarum (KC426951) on growth and innate responses of Nile tilapia Oreochromis niloticus were evaluated in biofloc technology system and stagnant-renewal culture system (SRCS). The 90-day-long experiment contained four treatments: SRCS without probiotic (T1), SRCS with probiotic (T2), biofloc without probiotic (T3), and biofloc with probiotic (T4). The administration dose of probiotic was 2 × 10 CFU kg diet.

Prevalence of Substance Abuse (Tobacco, Alcohol, Narcotics and Psychotropic Drugs) and Its Relationship to Family Factors in Pre-university Male Students in Shiraz 2017-2018.

Teenagers are valuable resources in communities and they are faced with multiple risk factors. Factors such as family attachment, devotion to family, parent's educational level, and parental support are the protective factors against high-risk behaviors. The purpose of this study was to determine the prevalence of drug use among pre-university students and its relationship with familial factors.

DNA damage, acetylcholinesterase activity, and hematological responses in rainbow trout exposed to the organophosphate malathion.

Effects of sub-lethal concentrations (0 (control), 0.009, 0.014, and 0.

Effects of chronic lead and cadmium exposure on the oriental river prawn (Macrobrachium nipponense) in laboratory conditions.

During the past decade, the total population and overall reproductive performance of the oriental river prawn (Macrobrachium nipponense) in the Anzali international wetland and its basin rivers has been severely reduced. This seems to be caused by an increase in heavy metal pollution from industrial wastewaters. We investigated the effects of chronic levels of lead (Pb) and cadmium (Cd) on reproductive success, hemato-immunology, crustacean hyperglycemic hormone (cHH), Pb/Cd bioaccumulation and histopathology of hepatopancreas of oriental river prawn.

Droplet vitrification versus straw cryopreservation for spermatozoa banking in Persian sturgeon (Acipenser persicus) from metabolite point of view.

Persian sturgeon (Acipenser persicus), a commercially valuable and critically endangered fish species has been suffering considerable declines in populations in the nature due to over-fishing, habitat destruction and marine pollution during past decades. Since there were no achievements in artificial reproduction programs, genetic resource banking such as gametes and embryo cryopreservation can be a good strategy however, reported resulting gamete qualities were considerably low. In the present study, the metabolome content of Persian sturgeon spermatozoa was investigated during common straw cryopreservation and novel droplet vitrification by the use of H NMR (Nuclear Magnetic Resonance) spectroscopy.

Characterization of Gamma-Irradiated L. (Rosemary).

Objectives: L., a member of the family Lamiaceae, is regarded as the spice with the highest antioxidant activity.

Materials And Methods: In this study, the transmission electron microscopy, X-ray diffraction, and fourier transform infrared spectroscopy (FTIR) physicochemical characteristics of the nanostructure of gamma-irradiated rosemary were investigated.

H NMR metabolic profiling of the cryopreserved spermatozoa of the wild endangered Persian sturgeon (Acipenser persicus) with the use of beta-cyclodextrin as an external cryoprotectant.

Persian sturgeon (Acipenser persicus) is an endangered species and genetic resource banking such as gametes and embryo preservation could be one of the most pursued conservation approaches. In this study, deleterious effects of the traditional cryopreservation technique and the effect of different doses of 2-hydroxypropyl-beta-cyclodextrin (HβCD) on thawed spermatozoa quality (motility duration and percentage) of Persian sturgeon (Acipenser persicus) were investigated from metabolic aspects of view. For cryopreserving, semen was diluted with Tris-HCl (100 mM) extenders containing 0, 5, 10, and 15 mM of HβCD in a ratio of 1:1 (semen/extenders).

The effects of specific expression of apoptin under the control of PSES and PSA promoter on cell death and apoptosis of LNCaP cells.

Objectives: Apoptotic effect of apoptin has been demonstrated in numerous studies. However, its tumor specificity has been questioned by some reports. The aim of this study was to confine the expression of apoptin in the prostate tumor cells by inducing its gene expression under the control of a chimeric enhancer composing of prostate-specific membrane antigen (PSMA) and prostate-specific antigen (PSA) regulatory elements (PSES).

Minimal methylation classifier (MIMIC): A novel method for derivation and rapid diagnostic detection of disease-associated DNA methylation signatures.

Rapid and reliable detection of disease-associated DNA methylation patterns has major potential to advance molecular diagnostics and underpin research investigations. We describe the development and validation of minimal methylation classifier (MIMIC), combining CpG signature design from genome-wide datasets, multiplex-PCR and detection by single-base extension and MALDI-TOF mass spectrometry, in a novel method to assess multi-locus DNA methylation profiles within routine clinically-applicable assays. We illustrate the application of MIMIC to successfully identify the methylation-dependent diagnostic molecular subgroups of medulloblastoma (the most common malignant childhood brain tumour), using scant/low-quality samples remaining from the most recently completed pan-European medulloblastoma clinical trial, refractory to analysis by conventional genome-wide DNA methylation analysis.

Effects of the prebiotic mannan-oligosaccharide on the stress response of feed deprived zebrafish (Danio rerio).

Feed deprivation has deleterious effects on fish behavior and stress physiology which may susceptible them to disease outbreak. Functional ingredients in diets may substantially impact the physiology and stress responses of host organisms. Here, we hypothesized that the administration of a dietary prebiotic might attenuate the negative influences of feed deprivation on the behavioral profile of anxiety and physiological responses to stress in zebrafish (Danio rerio).

Novel molecular subgroups for clinical classification and outcome prediction in childhood medulloblastoma: a cohort study.

Background: International consensus recognises four medulloblastoma molecular subgroups: WNT (MB), SHH (MB), group 3 (MB), and group 4 (MB), each defined by their characteristic genome-wide transcriptomic and DNA methylomic profiles. These subgroups have distinct clinicopathological and molecular features, and underpin current disease subclassification and initial subgroup-directed therapies that are underway in clinical trials. However, substantial biological heterogeneity and differences in survival are apparent within each subgroup, which remain to be resolved.

Metabolic changes in droplet vitrified semen of wild endangered Persian sturgeon Acipenser persicus (Borodin, 1997).

Comparative quantitative metabolite profiling can be used for better understanding of cell functions and dysfunctions in particular circumstances such as sperm banking which is an important approach for cryopreservation of endangered species. Cryopreservation techniques have some deleterious effects on spermatozoa which put the obtained results in controversy. Therefore, in the present study, quantitative H NMR (Nuclear Magnetic Resonance) based metabolite profiling was conducted to evaluate metabolite changes related to energetics and some other detected metabolites in vitrified semen of critically endangered wild Acipenser persicus.

Androgen receptor blockade using flutamide skewed sex ratio of litters in mice.

Maternal testosterone has been indicated to affect sex ratio of offspring. The present study was conducted to elucidate the role of androgen receptor in this regard by blockade of androgen receptor using flutamide in female mice. Mice were randomly assigned to two experimental groups.

Influence of supplemented diet with Pediococcus acidilactici on non-specific immunity and stress indicators in green terror (Aequidens rivulatus) during hypoxia.

Green terror fish were fed for two months with three types of feed including: control diet (C), diet containing 2% fish oil (O) and diet containing fish oil supplemented with 0.1% Pediococcus acidilactici bacteria (PA). At the end of the feeding period, 50 fish of different groups (n = 3) with an average weight of 4.