A solid-liquid microextraction approach from a spot in an adsorbent layer of a chromatographic plate.

A new approach to extracting substances from a spot on a chromatographic plate for subsequent liquid chromatography-mass spectrometry analysis is described. This method involves extraction in a solid phase (an adsorbent layer of a chromatographic plate) - a liquid system using a simple device. For a single extraction of six selected coccidiostats from the adsorbent layer on the chromatographic plate with silica gel, 50 µL of methanol was used for 5 min.

Some theoretical considerations on preparative separation with orthogonal pressurised planar electrochromatography.

In this work, separation of multicomponent mixtures containing components with the same and different electrophoretic mobility by using orthogonal pressurised planar electrochromatography is studied theoretically. Additionally, a simple way for determination of a maximum amount of mixture causing volume overload of flat/planar columns used in this technique is presented. In the next stage, effects of change in different parameters on process performance by simulation case studies are investigated.

Preliminary results for interval feeding the orthogonal pressurized planar electrochromatography system with sample solution for its preparative separation.

The orthogonal pressurized planar electrochromatography (OPPEC) is an example of 2-D separation technique, in which two simultaneous and orthogonal processes of electrophoresis and chromatography are involved in the separation mechanism. In the case of preparative separation of substances characterized by different electrophoretic mobility, such separation system can be constantly fed with the sample solution and the separated components can be constantly collected at its outlet. In the paper, as opposed to the previous studies, we discuss the capabilities of OPPEC technique for preparative separation of substances characterized by the same electrophoretic mobility.

Influence of some operation variables on continuous separation process of orthogonal pressurized planar electrochromatography.

Orthogonal pressurized planar electrochromatography (OPPEC) is the new separation technique, in which two simultaneous and orthogonal processes of electrochromatography/electrophoresis and chromatography proceed under external pressure. It means in the OPPEC separation system a mobile phase solution hydrodynamically flows against adsorbent layer of the chromatographic plate and electric field is orthogonally applied to it. This technique can be applied to analytical and preparative separations.