Electrochemical detection in a paper-based separation device.

Prototypes of microfluidic paper-based separation devices with amperometric detection were developed and evaluated. Photolithography was used to make a gold electrochemical microcell on polyester and that microcell was coupled to a strip of paper where a chromatographic separation occurs. The device performance was demonstrated with the separation and quantification of uric and ascorbic acid in mixtures.

Electrochemical detection of cysteine in a flow system based on reductive desorption of thiols from gold.

A simple strategy for cysteine determination using flow-injection analysis with electrochemical detection is described. The approach is based on the chemisorptions reactions of the sulfur moiety of cysteine upon polycrystalline gold electrodes and its subsequent reductive desorption. The electrochemical measurements were accomplished by the application of differential pulse voltammetry (DPV) for the operational optimization and pulsed electrochemical detection (PED) in combination with flow-injection analysis for the electrochemical detection as time function.