Publications by authors named "Rafael Vilhena Reis Neto"

Identifying and counting fish are crucial for managing stocking, harvesting, and marketing of farmed fish. Researchers have used convolutional networks for these tasks and explored various approaches to enhance network learning. Batch normalization is one technique that improves network stability and accuracy.

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Recent years have witnessed a remarkable global surge in fish production, with Nile tilapia (Oreochromis niloticus) emerging as a prominent contributor owing to its high demand as a nutritious food source. However, unlike terrestrial species, maintaining genealogical control and collecting phenotypic data in fish farming poses significant challenges, necessitating advancements to support genetic improvement programmes. While conventional methods, such as body measurements using rulers and photographs are prevalent in data collection, the potential of ultrasound-a less invasive and efficient tool for fish measurement-remains underexplored.

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Background: The myostatin gene has played an important role in the genetic improvement of the main species of economic importance; however, it has not yet been described for some Neotropical fish essential for aquaculture. This study aimed to characterize the myostatin gene of pacu, Piaractus mesopotamicus, and investigate the association of a microsatellite marker in this gene with the weight of fish.

Methods And Results: The myostatin gene sequence was obtained after following a RACE-PCR strategy based on a partial mRNA sequence available in the GenBank database and the alignment of myostatin sequences from other fish species.

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Article Synopsis
  • The study compared the growth performance of pacu, tambaqui, and their hybrids (tambacu and paqui) across different feeding programs with varying crude protein levels.
  • Researchers used 30 fish from each species, placing them in cages for a 5-month period where they were weighed six times.
  • Findings showed that while pure pacu had a higher growth rate, tambacu achieved a greater final weight, and lower protein diets surprisingly correlated with better growth, suggesting longer studies are needed to confirm these results.
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  • Red tilapia, valued for its appealing coloration, suffers in market value due to black blotches on their skin, which negatively impact selective breeding efforts for blemish-free fish.
  • The study investigated how these blotches affect growth rates and body measurements in various genetic groups of red tilapia over 250 days, using path analysis to determine the relationships between traits.
  • Findings showed high correlation among body traits while revealing that black blotches have minimal direct and indirect effects on growth, suggesting the potential for breeding high-performance red tilapia without blemishes.
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This study aims to investigate the effect of different cooling rates on the semen cryopreservation of curimba (Prochilodus lineatus). Nineteen ejaculates were obtained from adults males and cryopreserved at 15°C/min (CR15), 30°C/min (CR30) (controlled temperature inside and outside straw, speed was stable during freezing) and direct freezing in liquid nitrogen vapour (~35.6°C/min) (CRNV).

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Growth is one of the most important traits from both a physiological and economic perspective in aquaculture species. Thus, identifying the genomic regions and genes underpinning genetic variation for this trait is of particular interest in several fish species, including rainbow trout. In this work, we perform a genome-wide association study (GWAS) to identify the genomic regions associated with body weight at tagging (BWT) and at 18 months (BW18M) using a dense SNP panel (57 k) and 4596 genotyped rainbow trout from 105 full-sib families belonging to a Chilean breeding population.

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