Symbiotic nitrogen fixation (SNF) profoundly alters plant and bacteroid metabolism; however, SNF impact on folates and one-carbon (1C) metabolism are unknown. To explore this, SNF was induced in Phaseolus Vulgaris with Rhizobium etli. Nodules accumulated the highest folate concentration yet reported in a plant tissue (60 nmol/g fresh weight).
View Article and Find Full Text PDFTransient in planta transformation is a fast and cost-effective alternative for plant genetic transformation. Most protocols for in planta transformation rely on the use of Agrobacterium-mediated transformation. However, the protocols currently in use are standardized for small-sized plants due to the physical and economic constraints of submitting large-sized plants to a vacuum treatment.
View Article and Find Full Text PDFOne of the initial and critical procedures for the analysis of metabolomics data using liquid chromatography and mass spectrometry is feature detection. Feature detection is the process to detect boundaries of the mass surface from raw data. It consists of detected abundances arranged in a two-dimensional (2D) matrix of mass/charge and elution time.
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