Publications by authors named "Rafael P Madeira"

Article Synopsis
  • Parasites cause many neglected tropical diseases, affecting over a billion people and leading to millions of deaths each year.
  • Recent research shows that tiny particles called extracellular vesicles (EVs) from these parasites help them survive by interacting with host cells and spreading drug resistance.
  • The paper suggests better methods to study these EVs, including how to collect and understand them from infected people and animals, and talks about different tests that can be done in host cells.
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Extracellular vesicles (EVs) are lipid bilayer envelopes that encapsulate cell-specific cargo, rendering them promising biomarkers for diverse diseases. Chagas disease, caused by the parasite , poses a significant global health burden, transcending its initial epicenter in Latin America to affect individuals in Europe, Asia, and North America. In this study, we aimed to characterize circulating EVs derived from patients with chronic Chagas disease (CCD) experiencing a reactivation of acute symptoms.

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Extracellular vesicles (EVs) are lipid bilayer envelopes that encase several types of molecules. Their contents mostly reflect their cell origin and possible targets at other locations in the organism and can be modified in pathological conditions to interfere with intercellular communication, thus promoting disease establishment and development. These characteristics, in addition to their presence in virtually all body fluids, make such vesicles ideal for biomarker discovery in human diseases.

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Chagas disease, a neglected tropical disease (NTD) caused by the flagellated protozoan (), is a major public health problem. It was initially restricted to Latin America, but it is now expanding globally. Host and pathogen interactions are crucial in the establishment of disease, and since 1970, it has been known that eukaryotic cells release extracellular vesicles (EVs), which in turn have an important role in intercellular communication in physiological and pathological conditions.

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Purpose: We analyzed the frequency, viability, and genetic characteristics of T. gondii in pork heart samples.

Methods: Thirty-five fresh pork samples were purchased in a slaughterhouse in Erechim city.

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Ergosterol biosynthesis inhibitors, such as posaconazole and ravuconazole, have been proposed as drug candidates for Chagas disease, a neglected infectious tropical disease caused by the protozoan parasite Trypanosoma cruzi. To understand better the mechanism of action and resistance to these inhibitors, a clone of the T. cruzi Y strain was cultured under intermittent and increasing concentrations of ravuconazole until phenotypic stability was achieved.

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