Publications by authors named "Rafael Maura Perez"
Effective drug delivery to the central nervous system (CNS) remains a challenge due to the blood-brain barrier (BBB). Macromolecules such as proteins and peptides are unable to cross BBB and have poor therapeutic efficacy due to little or no drug distribution. A promising alternative is the conjugation of a drug to a shuttle molecule that can reach the CNS via receptor-mediated transcytosis (RMT).
View Article and Find Full Text PDFIntroduction: Proper conformational arrangement of the E2 molecules of bovine viral diarrhoea-mucosal disease virus (BVD-MDV) is crucial to obtain an effective recombinant vaccine candidate against the disease. In this study, we characterised a new molecule composed of two distinct sequences of the E2 glycoprotein of BVD-MDV and the Fc fragment of human immunoglobulin (BVDE2Fc).
Materials And Methods: The chimaeric protein was expressed in mammalian cell lines of different species by adenoviral transduction and purified by immobilised metal-affinity chromatography.
Article Synopsis
- Recombinant erythropoietin (EPO) is used for treating anemia and chronic renal failure, and newer long-acting variants have been developed for less frequent dosing through modifications like glycosylation.
- High levels of EPO were successfully produced in goat's milk using an adenoviral vector, showing that the molecular weight of EPO-Fc affects its hematopoietic activity.
- The study concludes that the types of sugars (sialylated N-glycans) attached to EPO-Fc significantly influence its biological activity in the body, more than just its molecular weight.