Effects of atrazine, diuron and glyphosate mixtures on zebrafish embryos: acute toxicity and oxidative stress responses.

Synthetic pesticides are known for their toxic effects on non-target aquatic organisms. However, little is known about their effects when present in mixtures, which are closer to realistic exposure scenarios. Therefore, this study evaluates the toxicity of pesticides such as diuron, atrazine and glyphosate, individually and in combination, in zebrafish embryos, investigating their mechanisms of oxidative stress.

Advances in green carbon-based biosorbents: From conventional to miniaturized sample preparation strategies.

Developing novel sorbent phases has advanced solid-based sample preparation techniques, improving analytical performance in complex matrices. Carbon-based sorbents, known for their high surface area, thermal and mechanical stability, and modifiability due to abundant organic functional groups, have emerged as exceptional materials in this field. Due to their versatile characteristics, carbon-based materials have been extensively investigated as promising materials for anchoring and functionalization with biopolymers, resulting in innovative hybrid materials, so-called carbon-based biosorbents.

HUMAP: Hierarchical Uniform Manifold Approximation and Projection.

Dimensionality reduction (DR) techniques help analysts to understand patterns in high-dimensional spaces. These techniques, often represented by scatter plots, are employed in diverse science domains and facilitate similarity analysis among clusters and data samples. For datasets containing many granularities or when analysis follows the information visualization mantra, hierarchical DR techniques are the most suitable approach since they present major structures beforehand and details on demand.

Lifetime sexual violence among transgender women and travestis (TGW) in Brazil: Prevalence and associated factors.

Objective: To describe the prevalence, characteristics, and factors associated with sexual violence in transgender women and travestis (TGW) in Brazil.

Methods: This cross-sectional study was conducted in five Brazilian cities (Campo Grande, Manaus, Porto Alegre, Salvador, and São Paulo) between 2019 and 2021. Participants were recruited using the respondent-driven sampling (RDS) technique.

Advances and Applications of Hybrid Graphene-Based Materials as Sorbents for Solid Phase Microextraction Techniques.

The advancement of traditional sample preparation techniques has brought about miniaturization systems designed to scale down conventional methods and advocate for environmentally friendly analytical approaches. Although often referred to as green analytical strategies, the effectiveness of these methods is intricately linked to the properties of the sorbent utilized. Moreover, to fully embrace implementing these methods, it is crucial to innovate and develop new sorbent or solid phases that enhance the adaptability of miniaturized techniques across various matrices and analytes.

Disentangling doping and strain effects at defects of grown MoS monolayers with nano-optical spectroscopy.

The role of defects in two-dimensional semiconductors and how they affect the intrinsic properties of these materials have been a widely researched topic over the past few decades. Optical characterization techniques such as photoluminescence and Raman spectroscopies are important tools to probe the physical properties of semiconductors and the impact of defects. However, confocal optical techniques present a spatial resolution limitation lying in a μm-scale, which can be overcome by the use of near-field optical measurements.

Biochemical Markers for Liver Injury in Zebrafish Larvae.

Liver plays a crucial role in detoxification processes and metabolism of xenobiotics, and therefore, it is a target organ of toxicity of different classes of chemicals. In this context, some key enzymes present in liver are considered to be good biochemical markers of hepatic damage and can have their activities determined via spectrophotometry. Aspartate and alanine aminotransferases, alkaline phosphatase, lactate dehydrogenase, and glutathione peroxidase are enzymes that have activities often changed in response to hepatotoxic compounds and can be accessed through the larval period of zebrafish (Danio rerio).

2,4-D Herbicide-Induced Hepatotoxicity: Unveiling Disrupted Liver Functions and Associated Biomarkers.

2,4-dichlorophenoxyacetic acid (2,4-D) is a widely used herbicide worldwide and is frequently found in water samples. This knowledge has prompted studies on its effects on non-target organisms, revealing significant alterations to liver structure and function. In this review, we evaluated the literature on the hepatotoxicity of 2,4-D, focusing on morphological damages, toxicity biomarkers and affected liver functions.

How the Adequate Choice of Plant Species Favors the Restoration Process in Areas Susceptible to Extreme Frost Events.

This work aimed to evaluate the impacts caused by extreme frost events in an ecological restoration area. We grouped the species in three ways: (1) type of trichome coverage; (2) shape of the seedling crown; and (3) functional groups according to the degree of damage caused by frost. The variables of the restored area and species characteristics were selected to be subjected to linear generalization analysis models (GLMs).

Molecularly imprinted polymers in online extraction liquid chromatography methods: Current advances and recent applications.

Background: One of the primary objectives in green analytical practices is the seamless integration of extraction and separation steps, resulting in the augmentation of both analytical throughput and method performance. Consequently, the exploration of prospective sorbent materials has drawn significant attention in the scientific community, particularly concerning the potential for online procedures. Employing the optimal sorbent material within an automated analytical approach holds the promise of elevating the precision of the analytical evaluation.

Chemical Composition, In Vitro Antitumor Effect, and Toxicity in Zebrafish of the Essential Oil from (L.) Cronquist (Asteraceae).

The essential oil from (Asteraceae) aerial parts (CBEO) was extracted by hydrodistillation in a Clevenger-type apparatus and was characterized by gas chromatography-mass spectrometry. The antitumor potential was evaluated against human tumor cell lines (melanoma, cervical, colorectal, and leukemias), as well as non-tumor keratinocyte lines using the MTT assay. The effect of CBEO on the production of Reactive Oxygen Species (ROS) was evaluated by DCFH-DA assay, and a protection assay using the antioxidant N-acetyl-L-cysteine (NAC) was also performed.

A Grid-Based Method for Removing Overlaps of Dimensionality Reduction Scatterplot Layouts.

Dimensionality Reduction (DR) scatterplot layouts have become a ubiquitous visualization tool for analyzing multidimensional datasets. Despite their popularity, such scatterplots suffer from occlusion, especially when informative glyphs are used to represent data instances, potentially obfuscating critical information for the analysis under execution. Different strategies have been devised to address this issue, either producing overlap-free layouts that lack the powerful capabilities of contemporary DR techniques in uncovering interesting data patterns or eliminating overlaps as a post-processing strategy.

Influence of Restorative Material on the Distribution of Loads to the Bone in Hybrid Abutment Crowns-In Vitro Study.

: The objective of this study was to evaluate the load transmitted to the peri-implant bone by seven different restorative materials in single-unit rehabilitations with morse taper implants using a strain gauge. : In a polyurethane block that simulated type III bone, a morse taper platform implant was installed (3.5 × 11 mm) in the center and 1 mm below the test base surface, and four strain gauges were installed around the implant, simulating the mesial, distal, buccal and lingual positions.

Detection of Failures in Metal Oxide Surge Arresters Using Frequency Response Analysis.

This work presents an innovative application of Frequency Response Analysis (FRA) in order to detect early degradation of Metal Oxide Surge Arresters (MOSAs). This technique has been widely used in power transformers, but has never been applied to MOSAs. It consists in comparisons of spectra, measured at different instants of the lifetime of the arrester.

Desorption electrospray ionization and matrix-assisted laser desorption/ionization as imaging approaches for biological samples analysis.

The imaging of biological tissues can offer valuable information about the sample composition, which improves the understanding of analyte distribution in such complex samples. Different approaches using mass spectrometry imaging (MSI), also known as imaging mass spectrometry (IMS), enabled the visualization of the distribution of numerous metabolites, drugs, lipids, and glycans in biological samples. The high sensitivity and multiple analyte evaluation/visualization in a single sample provided by MSI methods lead to various advantages and overcome drawbacks of classical microscopy techniques.

A Remote Monitoring Platform for the Management of Lower Limb Vascular Diseases.

This short paper describes a remote monitoring platform proposed in the Inno4health project. The platform aims to guide patients and clinicians during the treatment of lower limb vascular disorders, namely, to correct abnormal foot pressure and temperature to prevent diabetic foot ulcers and to monitor interface pressure, leg position and elevation for venous ulcers patients.

Marker events associated with adherence to HIV/AIDS treatment in a cohort study.

Objective: To analyze how clinical and social events may impact adherence to antiretroviral treatment for HIV.

Methods: This is a historical cohort study with 528 patients who underwent treatment for HIV in a specialized care service in Alvorada, RS. A total of 3429 queries executed between the years 2004 and 2017 were analyzed.

The Flow of Trust: A Visualization Framework to Externalize, Explore, and Explain Trust in ML Applications.

We present a conceptual framework for the development of visual interactive techniques to formalize and externalize trust in machine learning (ML) workflows. Currently, trust in ML applications is an implicit process that takes place in the user's mind. As such, there is no method of feedback or communication of trust that can be acted upon.

A review on the ecotoxicity of macrocyclic lactones and benzimidazoles on aquatic organisms.

Despite its wide production and several applications, veterinary antiparasitics from macrocyclic lactones and benzimidazole classes have not received much scientific attention concerning their environmental risks. Thus, we aimed to provide insights into the state of the environmental research on macrocyclic lactone and benzimidazole parasiticides, emphasizing their toxicity to non-target aquatic organisms. We searched for relevant information on these pharmaceutical classes on PubMed and Web of Science.

Palliative therapy for malignant gastric outlet obstruction: how does the endoscopic ultrasound-guided gastroenterostomy compare with surgery and endoscopic stenting? A systematic review and meta-analysis.

Introduction: The gold-standard procedure to address malignant gastric outlet obstruction (MGOO) is surgical gastrojejunostomy (SGJJ). Two endoscopic alternatives have also been proposed: the endoscopic stenting (ES) and the endoscopic ultrasound-guided gastroenterostomy (EUS-G). This study aimed to perform a thorough and strict meta-analysis to compare EUS-G with the SGJJ and ES in treating patients with MGOO.

Randomized Clinical Trial Comparing Insertion Torque and Implant Stability of Two Different Implant Macrogeometries in the Initial Periods of Osseointegration.

Objectives: The present study compared two implants with different macrogeometries placed in healed alveolar sites, evaluating the insertion torque (ITV) and implant stability quotient (ISQ) values at three different periods. Methods: Seventy patients with a total of 100 dental implants were allocated into two groups (n = 50 per group): DuoCone implants (DC group) that included 28 implants in the maxilla and 22 in the mandible, and Maestro implants (MAE group) that included 26 in the maxilla and 24 in the mandible. The ITV was measured during the implant placement, and the ISQ values were measured immediately at implant placement (baseline) and after 30 and 45 days.

Atlantic flower-invertebrate interactions: A data set of occurrence and frequency of floral visits.

Encounters between flowers and invertebrates are key events for the functioning of tropical forests. Assessing the structure of networks composed of the interactions between those partners leads to a better understanding of ecosystem functioning and the effects of environmental factors on ecological processes. Gathering such data is, however, costly and time-consuming, especially in the highly diverse tropics.

Electromembrane Extraction of Naphthenic Acids in Produced Water Followed by Ultra-High-Resolution Mass Spectrometry Analysis.

Naphthenic acids comprise one of the most toxic compounds of the produced water released from offshore oil platforms. Therefore, developing and applying faster, simpler, and more efficient analytical methods for analyzing naphthenic acids are urgently needed. Electromembrane extraction (EME) uses the electrokinetic migration of target ions through a porous membrane.

Acute Hemorrhagic Leukoencephalopathy Triggered by COVID-19 Infection.

This study represents an additional case of a rare entity and complication of COVID-19. To further describe COVID's association with acute hemorrhagic leukoencephalopathy (AHL), a variant of acute disseminated encephalomyelitis. Besides, subsequent neuropsychological evaluation is described.