Behind the Optimization of the Sensor Film: Bioconjugation of Triangular Gold Nanoparticles with Hemoproteins for Sensitivity Enhancement of Enzymatic Biosensors.

Electrochemical biosensors are widely used in a multitude of applications, such as medical, nutrition, research, among other fields. These sensors have been historically used and have not undergone many changes in terms of the involved electrochemical processes. In this work, we propose a new approach on the immobilization and enhancement of the electrochemical properties of the sensing layers through the control and bioconjugation of hemoproteins (hemoglobin, myoglobin, and cytochrome C) on anisotropic gold nanoparticles (gold nanotriangles (AuNTs)).

Surface Protection of Quaternary Gold Alloys by Thiol Self-Assembled Monolayers.

This work deals with a physical and chemical surface characterization of quaternary 18K, 14K, and 9K gold alloys and pure polycrystalline gold substrates. Surface microstructure and composition are evaluated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and X-ray fluorescence spectroscopy. Corrosion resistance of 18K gold alloys is explored by potentiodynamic polarization showing the influence of the manufacturing process on materials fabricated as plates and wires.

Distinct thermoresponsive behaviour of oligo- and poly-ethylene glycol protected gold nanoparticles in concentrated salt solutions.

In this work, the methoxy terminated oligo- and polyethylene glycol of different chain lengths (EG, = 7, 18, 45 and 136) is grafted on AuNP surfaces under conditions where they attain maximum grafting densities. These EG-AuNPs gain stability relative to the pristine c-AuNPs in aqueous solutions and in a wide temperature interval and they form stable suspensions in solutions of high NaCl concentrations. To show the thermoresponsive properties of these EG-AuNPs, temperature titration experiments are carried out in the presence of increasing amounts of salts.

Effective replacement of cetyltrimethylammonium bromide (CTAB) by mercaptoalkanoic acids on gold nanorod (AuNR) surfaces in aqueous solutions.

The highly packed cetyltrimethylammonium bromide (CTAB) bilayer built up on the surface of gold nanorods (AuNRs) when synthesized by the seed-mediated procedure hampers the complete ligand exchange under experimental conditions that preserves the stability of the dispersions. In the present work, a ligand exchange protocol by using carboxy-terminated alkanethiols of different chain lengths by means of a green approach that uses only aqueous solutions is presented. The protocol is based on the knowledge of the stability in the aqueous solution of both the starting CTAB-AuNRs and the final products that help in the choice of the experimental conditions used for ligand exchange.

Hemoglobin becomes electroactive upon interaction with surface-protected Au nanoparticles.

In this work, we report on the electrochemical behavior of bioconjugates prepared with gold nanoparticles (AuNP) capped with three different molecular layers (citrate anions, 6-mercaptopurine and ω-mercaptoundecanoic acid) and the protein hemoglobin (Hb). Freshly formed bioconjugates are deposited on a glassy carbon electrode and assayed for electroactivity. A pair of redox peaks with formal potential at -0.

Hemoglobin bioconjugates with surface-protected gold nanoparticles in aqueous media: The stability depends on solution pH and protein properties.

The identification of the factors that dictate the formation and physicochemical properties of protein-nanomaterial bioconjugates are important to understand their behavior in biological systems. The present work deals with the formation and characterization of bioconjugates made of the protein hemoglobin (Hb) and gold nanoparticles (AuNP) capped with three different molecular layers (citrate anions (c), 6-mercaptopurine (MP) and ω-mercaptoundecanoic acid (MUA)). The main focus is on the behavior of the bioconjugates in aqueous buffered solutions in a wide pH range.

Formation of a 1,8-octanedithiol self-assembled monolayer on Au(111) prepared in a lyotropic liquid-crystalline medium.

A characterization of the 1,8-octanedithiol (ODT) self-assembled monolayer (SAM) formed from a Triton X-100 lyotropic medium has been conducted by electrochemical techniques. It is found that an ODT layer of standing-up molecules is obtained at short modification time without removing oxygen from the medium. The electrochemical study shows that the ODT layer formed after 15 min of modification time has similar electron-transfer blocking properties to the layers formed from organic solvents at much longer modification times.

Functionalizing hydrogen-bonded surface networks with self-assembled monolayers.

One of the central challenges in nanotechnology is the development of flexible and efficient methods for creating ordered structures with nanometre precision over an extended length scale. Supramolecular self-assembly on surfaces offers attractive features in this regard: it is a 'bottom-up' approach and thus allows the simple and rapid creation of surface assemblies, which are readily tuned through the choice of molecular building blocks used and stabilized by hydrogen bonding, van der Waals interactions, pi-pi bonding or metal coordination between the blocks. Assemblies in the form of two-dimensional open networks are of particular interest for possible applications because well-defined pores can be used for the precise localization and confinement of guest entities such as molecules or clusters, which can add functionality to the supramolecular network.

Influence of the solution pH in the 6-mercaptopurine self-assembled monolayer (6MP-SAM) on a Au(111) single-crystal electrode.

Self-assembled monolayers (SAMs) of 6-mercaptopurine (6MP) have been prepared on a Au(111) single-crystal electrode by immersion of the metal surface in a 100 microM 6MP and 0.01 M HClO4 solution. The 6MP-SAM Au(111) single-crystal electrodes were transferred to the cell and allowed to equilibrate with the different aqueous working solutions before the electrochemical experiments.

Stabilization of gold nanoparticles by 6-mercaptopurine monolayers. Effects of the solvent properties.

6-Mercaptopurine-coated gold nanoparticles (6MP-AuNPs) have been prepared by modification of the nanoparticle surface with 6MP upon displacement of the protective layer of citrate anions. The modification has been studied by UV-vis and FTIR spectroscopies. A study of the stability of these 6MP-AuNPs in aqueous solutions as a function of ionic strength and pH has shown the importance of the charges on the stabilization.

Magnetic Langmuir-Blodgett films of ferritin with different iron contents.

Magnetic Langmuir-Blodgett films of four ferritin derivatives with different iron contents containing 4220, 3062, 2200, and 1200 iron atoms, respectively, have been prepared by using the adsorption properties of a 6/1 mixed monolayer of methyl stearate (SME) and dioctadecyldimethylammonium bromide (DODA). The molecular organization of the mixed SME/DODA monolayer is strongly affected by the presence of the water-soluble protein in the subphase as shown by pi-A isotherms, BAM images, and imaging ellipsometry at the water-air interface. BAM images reveal the heterogeneity of this mixed monolayer at the air-water interface.

Formation and dissolution processes of the 6-thioguanine (6TG) self-assembled monolayer. A kinetic study.

This is a report on the kinetics of the destruction and formation processes of the 6-thioguanine self-assembled monolayer (6TG SAM) on a mercury electrode from acid solutions by chronoamperometry. The destruction of the 6TG SAM that has been previously formed under open circuit potential conditions is carried out by stepping the potential from an initial value where the chemisorbed layer is stable up to potentials where the molecules are no longer chemisorbed. The destruction of the SAM has been described by a model that involves three types of contributions: (i) a Langmuir-type adsorption process, (ii) a 2D nucleation mechanism followed by a growth controlled by surface diffusion, and (iii) a 2D nucleation mechanism followed by a growth at a constant rate.