Publications by authors named "Rafael F Castaneda-Ruiz"

Plants play an important role in maintaining the ecological balance of the biosphere, but often suffer from pathogenic fungi during growth. During our continuing mycological surveys of plant pathogens from terrestrial plants in Jiangxi and Yunnan provinces, China, 24 strains of isolated from diseased and healthy tissues of plant leaves represented eight new species, viz. , , , , , , and .

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Article Synopsis
  • Research focuses on insect pathogenic fungi as eco-friendly alternatives to chemical insecticides, particularly in Cuba and Florida, where strains have been isolated from pests affecting coffee and sugar cane.* -
  • A newly developed PCR method using ribosomal intergenic spacer (rIGS) sequences proves effective in distinguishing species within a complex, and it is enhanced with additional genetic markers.* -
  • The findings characterize new fungal isolates associated with the coffee berry borer and propose these as a new taxon, demonstrating the utility of the rIGS-based PCR method for identifying new species in this context.*
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as a single genus in was introduced by morphological and phylogenetic analyses. Members of this genus occur mainly as asexual morphs, forming effuse, hairy colonies on decaying wood, plant stems, bamboo culms, and fallen leaves and shafts in terrestrial and freshwater habitats. In the present study, saprobic hyphomycetes from plant debris were investigated, and eight new species were introduced based on morphology and phylogenetic analyses of LSU, ITS, , and sequence data.

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Saprobic hyphomycetes are highly diverse on plant debris. Over the course of our mycological surveys in southern China, three new species, sp. nov.

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Three new species of , , and collected on dead branches of unidentified plants in Xishuangbanna, China, were proposed by morphological and molecular phylogenetic analysis. Phylogenetic analysis of the combined data of ITS-SSU-LSU-- sequences was performed using Maximum-Likelihood and Bayesian Inference, although and lack the sequences. Both molecular analyses and morphological data supported , and as three independent taxa within the Massarinaceae.

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Plant debris are habitats favoring survival and multiplication of various microbial species. During continuing mycological surveys of saprobic microfungi from plant debris in Yunnan Province, China, several Corynespora-like and Dendryphiopsis-like isolates were collected from dead branches of unidentified perennial dicotyledonous plants. Four barcodes, i.

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During an investigation of the diversity of aquatic hyphomycetes from southern China, two interesting isolates were collected. These two isolates were cultured and sequenced, and a BLAST search of their LSU sequences against data in GenBank revealed that the closest related taxa were in the genus Microthyrium. Phylogenetic analyses, based on the combined sequence data from the internal transcribed spacer (ITS) and large nuclear subunit ribosomal DNA (LSU), revealed that our isolates belong to the Microthyriaceae.

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The family Microthyriaceae is represented by relatively few mycelial cultures and DNA sequences; as a result, the taxonomy and classification of this group of organisms remain poorly understood. During the investigation of the diversity of aquatic hyphomycetes from southern China, several isolates were collected. These isolates were cultured and sequenced and a BLAST search of its LSU sequences against data in GenBank revealed that the closest related taxa are in the genus .

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Two isolates from Canada and the USA (UAMH 7122 and UAMH 7211, respectively) previously identified as were studied by morphology and six-locus phylogeny (, ITS, , , and ). UAMH 7122 and UAMH 7211 are morphologically related but phylogenetically distinct from (≡) and . Hence, UAMH 7122 and UAMH 7211 are described as a new species, sp.

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The asexual taxon , collected from submerged dicotyledonous leaves in Hainan, China, is described and illustrated herein. is characterized by macronematous, semimacronematous, septate and pigmented conidiophores and acrogenous, long lunate, vermiform to sigmoid, hyaline conidia with an eccentric basal appendage. Complete sequences of internal transcribed spacer (ITS) and partial sequences of nuclear large subunits ribosomal DNA (LSU) genes are provided.

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During a survey of endophytic fungi in aquatic plants collected from Tibet, PR China, a novel species, was isolated from . This novel species differs from other species of the genus by its finely verrucose, obovoid, ellipsoidal-obovoid and occasionally subglobose conidia. Phylogenetic analysis of the combined sequences of the internal transcribed spacers (ITS) and the translation elongation factor 1-alpha gene () confirmed that the isolated strain represents a member of the genus .

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Riparian forests are important to aquatic ecosystems and produce large quantities of organic matter that are recycled by the microbial community that includes microscopic fungi. The aim of this study was to unveil and compare the diversity of aquatic hyphomycetes associated to submerged leaf litter of tropical lotic and lentic environments in the Atlantic Forest of Northeast Brazil. Six sampling events were carried out in six points of two study areas: Biological Reserve "Mata da Chuva" (MC) and Environmental Protection Area "Lagoa da Mata" (LM), in Pernambuco, Brazil.

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A new genus and species, , is described and illustrated from leaves of mildewed tobacco. It is characterised by branched, septate hyphae from which arise macronematous, unbranched or spaced branched conidiophores and mono- or polyblastic conidiogenous cells that produced solitary and blastocatenate, obovoid, oblong, ellipsoidal, allantoid, broad fusiform to irregular, unicellular, hyaline conidia. The phylogenetic analyses, based on the combined sequence data from the small and large nuclear subunit ribosomal DNA (SSU and LSU), placed in the Leotiomycetes class, Helotiales order.

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Two new species of the genus , and , were described using combined morphological and DNA sequence data. The DNA sequences of respective strains including nuclear ribosomal DNA genes (nuSSU, ITS, nuLSU) and fragments of three protein-coding genes (ACT1, BT2, TEF1) were sequenced and compared with those from closely-related species to genera and (Family Sympoventuriaceae, Order Venturiales). Morphologically, both species showed typical ampulliform conidiophores and conidiogenous cells, features not seen in other species of .

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In a survey of soil and herbivore dung microfungi in Mexico and Spain, several dendryphiella-like species were found. Phylogenetic analyses based on ITS and LSU sequences showed that these fungi belonged to the family Dictyosporiaceae (Pleosporales) and represent an undescribed monophyletic lineage distant from . Therefore, the genus is proposed to accommodate three new species, , and .

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Article Synopsis
  • Two proposals have been put forward to allow DNA sequences to be used as types for naming certain fungi, which could fundamentally alter the definition of nomenclatural types and lead to various issues in scientific reproducibility and nomenclatural instability.
  • The authors argue against these proposals, suggesting that they would not effectively address the challenges of naming taxa based solely on DNA and propose instead that formulas for naming candidate taxa could be a better solution without changing existing nomenclature rules.
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Two interesting fungi belonging to the genus Cordana have been isolated recently in Spain from plant debris. Both are proposed here as new species, described and illustrated. Cordana mercadiana sp.

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During a study of the microfungi on decaying leaves collected in México we encountered a fungus sharing features of the anamorph genera Heliocephala and Holubovaniella. This hyphomycete resembled Hol. elegans in possessing obclavate to fusiform, three-septate, rostrate phragmoconidia, but it can be distinguished by its much shorter, branched, determinate conidiophores.

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Phaeobotrys gen. nov., based on Phaeodactylium acutisporum, characterized by branched and pigmented conidiophores, and the production of hyaline, appendiculate conidia from denticles on polyblastic conidiogenous cells which usually extend sympodially to form more conidiogenous loci, and Zanclospora stellata sp.

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