The efficacy of topical insulin application on rat model with burn wounds treated with adipose-derived stem cells.

Overall, major burn wounds may require special care and long-term hospitalization as they not only bring complications from the wound itself, but may also compromise the immune system, or even other organs. Previous studies have indicated that topical insulin cream shortened wound closure time in second-degree burns in rats. Transplanted adipose-derived stem cells (AD-MSCs) have been developed as an alternative to treat burns and to accelerate the healing process.

Dexmedetomidine and Bupivacaine Association in Caudal Epidural Injection in Mares.

The objective of the study was to compare the effects of caudal epidural bupivacaine and dexmedetomidine (DEX) combination, with bupivacaine or DEX plain for perineal analgesia in mares. Six healthy saddle mares weighing 330-370 kg and aged 10-15 years were used in this study. Each mare was assigned to receive three treatments: 0.

Welfare and pregnancy rate of ewes undergoing transcervical artificial insemination with ketamine subarachnoid anesthesia.

This study aimed to evaluate the impact of subarachnoid anesthesia with ketamine during transcervical artificial insemination (TCAI) on the welfare of ewes and on subsequent pregnancy rates. Ninety Suffolk adult ewes were randomized into three treatment groups: control group (CG), which underwent the TCAI procedure as established by cervical traction (CG; n = 30) and two groups that received subarachnoid anesthesia with ketamine at a dose of either 0.75 mg/kg (KE0.

Postoperative pain control in cats: clinical trials with pre-emptive lidocaine epidural co-administered with morphine or methadone.

Objectives The aim of the study was to evaluate the effectiveness of epidural lidocaine in combination with either methadone or morphine for postoperative analgesia in cats undergoing ovariohysterectomy. Methods Under general anesthesia, 24 cats that underwent ovariohysterectomy were randomly allocated into three treatment groups of eight each. Treatment 1 included 2% lidocaine (4.

Potentiation of epidural lidocaine by co-administering tramadol by either intramuscular or epidural route in cats.

This study investigated the analgesic and systemic effects of intramuscular (IM) versus epidural (EP) administration of tramadol as an adjunct to EP injection of lidocaine in cats. Six healthy, domestic, shorthair female cats underwent general anesthesia. A prospective, randomized, crossover trial was then conducted with each cat receiving the following 3 treatments: EP injection of 2% lidocaine [LEP; 3.

Efficacy of caudal epidural injection of lidocaine, xylazine and xylazine plus hyaluronidase in reducing discomfort produced by electroejaculation in bulls.

To test the hypothesis that epidural administration of lidocaine, xylazine or xylazine plus hyaluronidase provides reduced pain and stress during electroejaculation in bulls, eight 30-month-old Nellore bulls received saline solution (control), 2% lidocaine, 2% xylazine or 2% xylazine plus hyaluronidase injected into the first intercoccygeal (Co1-Co2) epidural space in randomized order. Heart rate, respiratory rate, mean arterial pressure, analgesia, animal behavior and motor blockade were evaluated before treatment and at predetermined intervals during and after treatment. Pain and stress were scored subjectively, and semen quality was evaluated.

Effects of epidural nalbuphine on intraoperative isoflurane and postoperative analgesic requirements in dogs.

Purpose: To measure the change in the minimum alveolar concentration of isoflurane (EtISO) associated with epidural nalbuphine and the postoperative analgesic requirements in dogs after ovariohysterectomy.

Methods: Twenty four healthy female dogs were randomly assigned to receive saline or nalbuphine at 0.3 or 0.

Efficacy of concurrent epidural administration of neostigmine and lidocaine for perineal analgesia in geldings.

Objective: To evaluate perineal analgesic effects of 3 doses of neostigmine coadministered epidurally with lidocaine to geldings.

Animals: 6 healthy geldings.

Procedures: A few days before each treatment, a catheter was inserted between the first and second coccygeal vertebrae via the caudal approach in each gelding; the catheter tip was threaded approximately 10 cm cranial into the midsacral region.

Scanning electron microscopy and microbiological evaluation of equine burn wound repair after platelet-rich plasma gel treatment.

Deep second-degree burns (DSDb) involve all epidermis layers, including the basal laminae. In general, DSDb heals with extensive areas of scarring. Platelet-rich plasma (PRP) is a source of growth factors, obtained by centrifugation and addition of thrombin and calcium, resulting in a gel.

Thoracic epidural analgesia via the lumbosacral approach using multiport catheters with a low concentration of bupivacaine and morphine in sheep.

Objective: To determine the analgesic and systemic effects of thoracic epidural administration of bupivacaine (BP) and morphine (MP) in conscious sheep.

Study Design: Randomized, crossover, experimental study.

Animals: Six healthy castrated sheep weighing between 40 and 50 kg.

Lumbosacral epidural magnesium prolongs ketamine analgesia in conscious sheep.

Purpose: To determine the analgesic, motor, sedation and systemic effects of lumbosacral epidural magnesium sulphate added to ketamine in the sheep.

Methods: Six healthy adult male mixed-breed sheep; weighing 43 ± 5 kg and aged 20-36 months. Each sheep underwent three treatments, at least 2 weeks apart, via epidural injection: (1) ketamine (KE; 2.

Evaluation of thoracic epidural analgesia induced by lidocaine, ketamine, or both administered via a lumbosacral approach in dogs.

Objective: To determine the analgesic and systemic effects of thoracic epidural administration of ketamine, lidocaine, or both in conscious dogs.

Animals: 6 adult mixed-breed dogs.

Procedures: Each dog received 2% lidocaine hydrochloride without epinephrine (3.

The efficacy and safety of levobupivacaine administered by lumbosacral epidural route in conscious sheep.

The aim of this study was to evaluate 3 doses of levobupivacaine (LB) epidurally administered in sheep. Six adult male 24-36 month-old sheep received levobupivacaine at 3 doses, LB05 (0.05 mg/kg), LB15 (0.

Segmental dorsolumbar epidural analgesia via the caudal approach using multiple port catheters with ketamine or lidocaine or in combination in cattle.

Objective: To determine the analgesic and systemic effects of epidural administration of ketamine, lidocaine or a combination of ketamine/lidocaine in standing cattle.

Study Design: Prospective, randomized, experimental trial.

Animals: Six healthy male cattle weighing between 335 and 373 kg.

Evaluation of two regional anesthetic methods on the front limb of dogs using hyperbaric bupivacaine.

Purpose: To evaluate the effects of bupivacaine 0.5 and 0.25% in intravenous regional anesthesia (IVRA) and brachial plexus block (BPB), respectively, on anesthesia, motor block and cardiovascular parameters in dogs.

Evaluation of segmental dorsolumbar epidural analgesia with ketamine hydrochloride administered by use of nonstyletted multiple-port catheters via the caudal approach in cattle.

Objective: To evaluate use of nonstyletted multiple-port catheters for epidural administration of ketamine hydrochloride via the caudal approach to induce analgesia of the paralumbar fossa (flank) in cattle.

Animals: 6 healthy bulls.

Procedures: 1 week before experiments began, a multiple-port catheter was inserted by use of a Tuohy needle in all cattle via the caudal approach (between the first and second coccygeal vertebrae); the tip was threaded approximately 48 cm cranial into the dorsolumbar region.

Effects of platelet-rich plasma gel on skin healing in surgical wound in horses.

Purpose: To establish a low-cost method to prepare platelet-rich plasma (PRP) and evaluates the potential of platelet derived factors to enhance wound healing in the surgical wounds in equine.

Methods: To obtain a PRP gel, calcium gluconate and autologous thrombin were added to platelet-rich plasma. For the tests six saddle horses were used and two surgical incisions were made in each animal.

Effects of caudal epidural ketamine and/or lidocaine on heifers during reproductive procedures: a preliminary study.

Six 20-24-month-old heifers received ketamine (KE), 2% lidocaine (Li), or ketamine and 2% lidocaine (KELI) into the first intercoccygeal (Co(1)-Co(2)) epidural space while standing. Heart rate, arterial blood pressure (systolic, diastolic, and mean), respiratory rate, rectal temperature, analgesia, sedation, and ataxia were determined before treatment and at predetermined intervals. Subjective scoring was used to assess pain and discomfort in reproductive manipulations.

Meperidine prolongs lidocaine caudal epidural anaesthesia in the horse.

The aim of the study was to evaluate and compare the effects of caudal epidural administration of meperidine (MP), lidocaine (LD), and a combination of the two (MPLD) in six mature saddle horses. Horses were randomly assigned to receive three treatments (MP 0.3 mg/kg; LD 0.

Subarachnoid butorphanol augments lidocaine sensory anaesthesia in calves.

To determine the efficacy and safety of subarachnoid butorphanol combined with lidocaine, six calves were studied. Each calf underwent two treatments, at least one week apart, via subarachnoid injection: (1) butorphanol (0.03 mg/kg) plus 2% lidocaine (4 mg/kg) and (2) 2% lidocaine (4 mg/kg) alone.

Perineal analgesia and hemodynamic effects of the epidural administration of meperidine or hyperbaric bupivacaine in conscious horses.

Epidural administration of bupivacaine and meperidine produces analgesia in several animal species and in humans. A prospective randomized study was conducted in 18 healthy horses to compare the effect of these 2 drugs administered by the epidural route. Animals were divided into 3 treatment groups of 6 animals each.

Analgesic and systemic effects of ketamine, xylazine, and lidocaine after subarachnoid administration in goats.

Objective: To determine the effects of ketamine hydrochloride, xylazine hydrochloride, and lidocaine hydrochloride after subarachnoid administration in goats.

Animals: 6 healthy goats.

Procedure: In each goat, ketamine (3 mg/kg), xylazine (0.