Chemical analysis and evaluation of antioxidant and antimicrobial activities of fruit fractions of L. f. (Arecaceae).

Background: Fruit consumption is currently considered beyond the nutritional aspects because of the important roles in disease prevention and benefits to health. The objective of this study was to characterize the chemical profile and evaluate the antioxidant and antimicrobial properties of different fractions obtained from fruit pulps of (MFFs).

Methods: Initially, chloroform, ethyl acetate and ethanol fractions were obtained from the pulps.

Development and validation of a rapid RP-HPLC-DAD analysis method for the quantification of pilocarpine in Pilocarpus microphyllus (Rutaceae).

A method using high performance liquid chromatography with diode array detector (HPLC-DAD) for identification and quantification of pilocarpine in the extract of Pilocarpus microphyllus, popularly known as jaborandi. The analysis was conducted using RP-18 column (250 mm × 4.6 mm x 5 μm id) and a buffer solution composed of acidified water, phosphoric acid and triethylamine and methanol as a mobile phase in a flow rate of 1.