Publications by authors named "Rafael A L Franco"
Article Synopsis
- Toehold switches are biosensors designed to detect specific RNAs, including those from viruses, with a programmable design process that allows for efficient engineering.* -
- They often face issues with leaky translation in their OFF state, which reduces their sensitivity and effectiveness as biosensors.* -
- A newly constructed serine integrase circuit significantly improves performance by minimizing leakage, increasing expression fold change, and lowering detection limits, making it easier to use in various biosensor applications.*
Bacillus subtilis employs five purine riboswitches for the control of purine de novo synthesis and transport at the transcription level. All of them are formed by a structurally conserved aptamer, and a variable expression platform harboring a rho-independent transcription terminator. In this study, we characterized all five purine riboswitches under the context of active gene expression processes both in vitro and in vivo.
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