The status of preimplantation genetic testing in the UK and USA.

Study Question: Has the number of preimplantation genetic testing (PGT) cycles in the UK and USA changed between 2014 and 2016?

Summary Answer: From 2014 to 2016, the number of PGT cycles in the UK has remained the same at just under 2% but in the USA has increased from 13% to 27%.

What Is Known Already: PGT was introduced as a treatment option for couples at risk of transmitting a known genetic or chromosomal abnormality to their child. This technology has also been applied as an embryo selection tool in the hope of increasing live birth rates per transfer.

Effect of methotrexate/vitamin B on DNA methylation as a potential factor in leukemia treatment-related neurotoxicity.

Unlabelled: Methotrexate (MTX) is administered to treat childhood acute lymphoblastic leukemia (ALL). It acts by inhibiting dihydrofolate reductase which reduces methyltetrahydrofolate, a key component in one carbon metabolism, thus reducing cell proliferation. Further perturbations to one carbon metabolism, such as reduced vitamin B levels via the use of nitrous oxide for sedation during childhood ALL treatment, may increase neurotoxicity risk.