Longitudinal Transcriptomic, Proteomic, and Metabolomic Response of to Inoculation of Liberibacter asiaticus.

Huanglongbing (HLB) is a fatal citrus disease that is currently threatening citrus varieties worldwide. One putative causative agent, Liberibacter asiaticus (Las), is vectored by , known as the Asian citrus psyllid (ACP). Understanding the details of Las infection in HLB disease has been hindered by its nature and the inability to confidently detect it in diseased trees during the asymptomatic stage.

Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample.

A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results.

Antibody-based PET of uPA/uPAR signaling with broad applicability for cancer imaging.

Mounting evidence suggests that the urokinase plasminogen activator (uPA) and its receptor (uPAR) play a central role in tumor progression. The goal of this study was to develop an 89Zr-labeled, antibody-based positron emission tomography (PET) tracer for quantitative imaging of the uPA/uPAR system. An anti-uPA monoclonal antibody (ATN-291) was conjugated with a deferoxamine (Df) derivative and subsequently labeled with 89Zr.

Discovery of Bifunctional Oncogenic Target Inhibitors against Allosteric Mitogen-Activated Protein Kinase (MEK1) and Phosphatidylinositol 3-Kinase (PI3K).

The synthesis of a series of single entity, bifunctional MEK1/PI3K inhibitors achieved by covalent linking of structural analogs of the ATP-competitive PI3K inhibitor ZSTK474 and the ATP-noncompetitive MEK inhibitor PD0325901 is described. Inhibitors displayed potent in vitro inhibition of MEK1 (0.015 < IC50 (nM) < 56.

Quantifying economic and environmental benefits of co-located firms.

Resource sharing among co-located firms--referenced in the industrial ecology literature as "industrial symbiosis"--engages traditionally separate industries in a collective approach to business and environmental management involving the physical exchanges of materials, energy, water, and byproducts. While industrial symbiosis is seen hypothetically as a win-win situation, there are few analyses of the economic and environmental consequences for the individual participants in multi-faceted exchanges. In this article, the nascent industrial symbiosis network in Guayama, Puerto Rico, is explored from environmental, economic, and regulatory perspectives of the individual participants and the community.