Publications by authors named "Rachel Joshi"
Article Synopsis
- - Mass spectrometry in chemoproteomics aims to directly identify where small molecules bind to target proteins, but it faces challenges like low protein abundance and difficulties in identifying modification sites with standard software.
- - Researchers explored BODIPY FL azide as a labeling reagent, utilizing its unique boron isotopes to develop a method that improves the detection of labeled peptides through a specific intensity ratio and mass defect filtering.
- - The study showed that using BODIPY for labeling not only increased peptide identification from complex samples but also offered a distinct isotopic signature, which could be useful for both UV and fluorescence detection methods.
Although heparan sulfate (HS) has been implicated in facilitating entry of enveloped viruses including herpes simplex virus (HSV), small molecules that effectively compete with this abundant, cell surface macromolecule remain unknown. We reasoned that entry of HSV-1 involving its glycoprotein D (gD) binding to HS could be competitively targeted through small, synthetic, nonsaccharide glycosaminoglycan mimetics (NSGMs). Screening a library of NSGMs identified a small, distinct group that bound gD with affinities of 8-120 nM.
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