Molecular shift register and its utilization as an autonomous DNA synthesizer.

A novel algorithmic approach to the synthesis of fairly long DNA molecules with nonrecurring sequences is demonstrated. The scheme exploits chemical embodiment of shift registers (SR) to execute algorithms similar to those used to generate pseudorandom numbers on a computer. Single stranded DNA molecules guide the synthesis of double stranded DNA according to the SR truth table.

CelI, a noncellulosomal family 9 enzyme from Clostridium thermocellum, is a processive endoglucanase that degrades crystalline cellulose.

The family 9 cellulase gene celI of Clostridium thermocellum, was previously cloned, expressed, and characterized (G. P. Hazlewood, K.

Sequence-specific molecular lithography on single DNA molecules.

Recent advances in the realization of individual molecular-scale electronic devices emphasize the need for novel tools and concepts capable of assembling such devices into large-scale functional circuits. We demonstrated sequence-specific molecular lithography on substrate DNA molecules by harnessing homologous recombination by RecA protein. In a sequence-specific manner, we patterned the coating of DNA with metal, localized labeled molecular objects and grew metal islands on specific sites along the DNA substrate, and generated molecularly accurate stable DNA junctions for patterning the DNA substrate connectivity.