Predatory bacteria prevent the proliferation of intraocular and fluoroquinolone-resistant .

Endogenous endophthalmitis caused by Gram-negative bacteria is an intra-ocular infection that can rapidly progress to irreversible loss of vision. While most endophthalmitis isolates are susceptible to antibiotic therapy, the emergence of resistant bacteria necessitates alternative approaches to combat intraocular bacterial proliferation. In this study the ability of predatory bacteria to limit intraocular growth of , , and was evaluated in a New Zealand white rabbit endophthalmitis prevention model.

Genome sequence generated by hybrid Nanopore-Illumina assembly of an extensively drug-resistant strain from a keratitis outbreak.

Here, we report the genome sequence of an extensively drug-resistant associated with a keratitis outbreak. This isolate was from the Center for Disease Control and Prevention and originates from a cornea. This sequence will facilitate studies of extensively drug resistant bacteria.

A rise in the frequency of mutant among keratitis isolates between 1993 and 2021.

Introduction: causes vision threatening keratitis. The transcription factor regulates virulence factors in response to the quorum sensing molecule N-3-oxo-dodecanoyl-L-homoserine lactone. isolates with mutations are characterized by an iridescent high sheen phenotype caused by a build-up of 2-heptyl-4-quinolone.

Intra-ocular Predation of Fluoroquinolone-Resistant and by Predatory Bacteria.

Endogenous endophthalmitis caused by Gram-negative bacteria is an intra-ocular infection that can rapidly progress to irreversible loss of vision. While most endophthalmitis isolates are susceptible to antibiotic therapy, the emergence of resistant bacteria necessitates alternative approaches to combat intraocular bacterial proliferation. In this study the ability of predatory bacteria to limit intraocular growth of , , and was evaluated in a New Zealand White rabbit endophthalmitis prevention model.

Rise in frequency of mutant among keratitis isolates between 1993 and 2021.

causes severe vision threatening keratitis. LasR is a transcription factor that regulates virulence associated genes in response to the quorum sensing molecule N-3-oxo-dodecanoyl-L-homoserine lactone. isolates with mutations are characterized by an iridescent high sheen phenotype caused by a build-up of 2-heptyl-4-quinolone.

IgaA Protein, GumB, Has a Global Impact on the Transcriptome and Surface Proteome of Serratia marcescens.

Bacterial stress response signaling systems, like the Rcs system are triggered by membrane and cell wall damaging compounds, including antibiotics and immune system factors. These regulatory systems help bacteria survive envelope stress by altering the transcriptome resulting in protective phenotypic changes that may also influence the virulence of the bacterium. This study investigated the role of the Rcs stress response system using a clinical keratitis isolate of Serratia marcescens with a mutation in the gene.

mCloverBlaster: A tool to make markerless deletions and fusions using lambda red and I-SceI in Gram-negative bacterial genomes.

This study introduces mCloverBlaster as a genetic tool to create deletions and transcriptional and translational fusions in bacterial genomes using recombineering. The major advantage of this system is that it can be used to make deletions and fusions without leaving a selectable marker on the chromosome. mCloverBlaster has a kanamycin resistance cassette with an I-SceI restriction site flanked by fragments of the gene for the mClover3 fluorescent protein including direct repeats of mClover3 sequence on both sides of the kanamycin resistance gene.