Publications by authors named "Rachel Bechar"
Nucleic Acids Res
November 2018
Article Synopsis
- - Genome editing of human induced pluripotent stem cells (iPSCs) is crucial for research in genetics, disease studies, and regenerative medicine, but low efficiency in CRISPR-Cas9 methods has been a barrier due to high cell death after electroporation.
- - Introducing BCL-XL significantly boosts iPSC survival and enhances genome editing success, with increases of 20- to 100-fold in gene insertion efficiency and up to 5-fold in gene deletion success.
- - Further use of the BCL inhibitor ABT-263 improves editing efficiency even more, enabling near-perfect knockout rates and making extensive genetic modifications in cell populations easier without the need for time-consuming individual cell cloning.
