Detection of spp. in milk from seronegative cows by real-time polymerase chain reaction in the region of Batna, Algeria.

Aim: The aim of this study was to detect spp. DNA in milk samples collected from seronegative cows using the real-time polymerase chain reaction (PCR) assay for diagnosis of brucellosis in seronegative dairy cows to prevent transmission of disease to humans and to reduce economic losses in animal production.

Materials And Methods: In this study, 65 milk samples were investigated for the detection of spp.