Alginate oligosaccharides can maintain activities of lysosomes under low pH condition.

The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides. Lysosome-alginate oligosaccharides composite were formulated for oral administration of lysosomes. The dissolution test confirmed the availability of the oral dosage form.

YPT7's deletion regulates yeast vacuoles' activity.

The yeast vacuole is functionally corresponding to vacuoles in eukaryote cells, it consists of a fusion protein that assists in the fusion of vacuoles and plays an important role in many processes. In addition, chemicals such as NHCl can reduce the size of vacuoles but as a side effect that also inhibits vacuoles making them inactive. In this study, to develop pre-treatments for extending the life of cut flowers, we constructed recombinant yeast using the fusion protein YPT7 and confirmed the activity of down-sized vacuoles.

Lysosomes Alteration in HeLa Cell Exposed with 1-Aminocyclopropane-1-Carboxylic Acid, the Ethylene Precursor of Plant Hormones.

The study of senescence preservative on cut flowers helps boost the commercial value of flowers. Senescence in cut flower is associated with an increase of ethylene production, and is significantly influenced by ethylene pathway. This study was conducted to investigate whether S-adenosyl-L-methionine (SAM) and aminocyclopropane-1-carboxylic acid (ACC) involved in the ethylene synthesis process are correlated with the lysosome.

Removal of Trans-2-nonenal Using Hen Egg White Lysosomal-Related Enzymes.

2-Nonenal is a long-chain aliphatic aldehyde containing nine carbons and an unsaturated bond. 2-Nonenal is the primary cause of odor associated with aging, with an unpleasant greasy and grassy odor. Lysosome, mitochondria, and peroxisome are significant organelles in eukaryotic cells that contain various hydrolases that degrade biomolecules.

The Response of Liposomes Modified with Lysosomal Membrane Proteins to the Targeting Ability Associated with Antimicrobial Activity.

Liposomes were modified using two different methods. In Method 1, liposomes were modified by mixing whole lysosomal proteins, lipid, and cholesterol before preparation. For Method 2, the liposomes were modified by mixing whole lysosomal proteins after liposome preparation.

Effects of Alginate Oligosaccharide Mixture on the Bioavailability of Lysozyme as an Antimicrobial Agent.

In this study, we report an oral drug delivery system without any additional process using pH-sensitive biopolymer, alginate, and alginate oligosaccharide with lysozyme as a model drug. These biopolymers exhibited pH-sensitive characteristics such as shrinking at acidic pH and eroding with dissolution at alkaline pH. The incorporation of lysozyme and biopolymers was performed an artificial intestinal juice (pH 6.

Relationships between blood Mg2+ and energy metabolites/enzymes after acute exhaustive swimming exercise in rats.

Magnesium (Mg) plays a central role in neuronal activity, cardiac excitability, neuromuscular transmission, muscular contraction, vasomotor tone, and blood pressure, all of which are significantly related to physical performance. To date, the available data about detection of blood total Mg (tMg; free-ionized, protein-bound, and anion-complex forms) are inconsistent, and there is limited information on blood free-ionized Mg (Mg(2+)) in relation to physical exercise. The aim of this study was to determine the biochemical changes related to energy metabolism after acute exhaustive swimming exercise (AESE) in rats in an attempt to correlate the role of blood Mg(2+) with metabolites/enzymes related to energy production.