A generalized system for automation of enzyme assays.

A flow system was developed, using a Technicon AutoAnalyzer, that is readily adaptable to a range of enzyme assays. The system includes lines for pumping substrate, cofactor, buffer and enzyme and for generating linear gradients. By using a variable-speed proportioning pump the incubation time may be continuously varied, and the system also allows for continuous variation in the pH, substrate or cofactor concentration, incubation temperature and enzyme concentration.

Further study of factors affecting amino acid incorporation into protein by isolated mitochondria.

1. The incorporation of amino acids into protein in isolated mitochondria has now been studied in more detail, and with mitochondria from a range of tissues. 2.

The effects of antibodies on cells. 3. Studies on the interaction of rat liver mitochondria and lysosomes with antibody and complement.

Complement-fixing and precipitating antibodies to mitochondrial and lysosomal fractions of rat liver were induced in rabbits. The incubation of these subcellular fractions with ferritin-labelled antibody revealed localization of the antibody on mitochondria and lysosomes, but no specific morphological effect of antibody and complement. Incubation of the cell fractions with antibody and complement did not impair the efficiency of mitochondrial oxidative phosphorylation or affect the latency and sedimentation characteristics of lysosomal acid phosphatase.

THE STIMULATION BY TREATMENT IN VIVO WITH TRI-IODOTHYRONINE OF AMINO ACID INCORPORATION INTO PROTEIN BY ISOLATED RAT-LIVER MITOCHONDRIA.

1. Normal and thyroidectomized rats were treated with near-physiological doses of tri-iodothyronine. Liver mitochondria were isolated and incubated with radioactive amino acids.