Cytotoxicity and DNA cleavage with core-shell nanocomposites functionalized by a KH domain DNA binding peptide.

A nanoconjugate was composed of metal oxide nanoparticles decorated with peptides and fluorescent dye and tested for DNA cleavage following UV light activation. The peptide design was based on a DNA binding domain, the so called KH domain of the hnRNPK protein. This "KH peptide" enabled cellular uptake of nanoconjugates and their entry into cell nuclei.

Eliminating tyrosine sequence variants in CHO cell lines producing recombinant monoclonal antibodies.

Amino acid sequence variants are defined as unintended amino acid sequence changes that contribute to product variation with potential impact to product safety, immunogenicity, and efficacy. Therefore, it is important to understand the propensity for sequence variant (SV) formation during the production of recombinant proteins for therapeutic use. During the development of clinical therapeutic products, several monoclonal antibodies (mAbs) produced from Chinese Hamster Ovary (CHO) cells exhibited SVs at low levels (≤3%) in multiple locations throughout the mAbs.

Increase of fluorescence anisotropy upon self-assembly in headgroup-labeled surfactants.

The change in fluorescence anisotropy upon micellization in headgroup-labeled surfactants is investigated. After eliminating the likelihood of depolarizing RET, anisotropy is shown to increase upon self-assembly due to increased rotational correlation times of the fluorophore. This is shown using two surfactant-fluorophore systems.

Echocardiographic assessment of the left ventricle in juvenile hypertension.

We studied with M-mode echocardiography the morphology and function of the left ventricle in a group of 36 juvenile hypertensives with borderline hypertension, whose cuff arm pressure exceeded 150/90 mmHg in at least three separate sessions. The results were compared with those of 23 age-matched normotensives with no evidence of any cardiovascular disease. Left ventricular hypertrophy (i.

Character of mitral valve flow in left atrial tumor.

The pulsed Doppler echocardiography (PDE) was used to evaluate the character of mitral valve flow in a large atrial tumor. The tumor obstructed the mitral orifice during diastole. PDE findings, however, showed normal triphasic diastolic flow within the actual mitral orifice and no changes typical for mitral stenosis.

Effect of verapamil on pulmonary hypertension and right ventricular hypertrophy induced in rats by intermittent high altitude hypoxia.

Adult male Wistar rats were used for studying the effect of Ca2+ antagonist verapamil on pulmonary hypertension, right ventricular hypertrophy and the medial thickness of pulmonary arterioles, induced by intermittent high altitude (IHA) hypoxia. This was simulated in a hypobaric chamber (7,000, 8 h daily, 5 days a week, 24 exposures). Verapamil was injected subcutaneously in a single dose of 8 mg/kg before each IHA exposure.

Changes of the right and left ventricles in rats exposed to intermittent high altitude hypoxia.

Significant structural, functional and metabolic differences between the right and left ventricles are present already in control animals. Intermittent high altitude (IHA) hypoxia (4 hr daily, 5 days a week, stepwise up to an altitude of 7000 m in a hypobaric chamber) induced in adult rats pulmonary hypertension and right ventricular hypertrophy; prolonged hypoxia also increased the relative left ventricular mass. Both chambers show proportionate increase in concentration of myofibrilar and collagenous proteins; the right-left difference, characteristic of animals living in normoxic environment, remains unaffected.

Intermittent high altitude hypoxia.

The effect of intermittent high altitude (IHA) hypoxia on the myocardium and lesser circulation was investigated in adult male Wistar rats. IHA can induce intermittent pulmonary hypertension and right ventricular hypertrophy in a relatively short time. Even marked pulmonary hypertension, right ventricular hypertrophy, and pulmonary vascular changes can be normalized when rats are removed from the hypoxic atmosphere.