Evolution of an idiotypic determinant: anti-Val.

An antibody population which reacts only with human sickle cell hemoglobin (HbS) and not with normal human hemoglobin, has been isolated from goat, sheep, and guinea pig antisera. These antibody populations termed anti-Val (Val), isolated from an individual goat (no. 6) and sheep (no.

Ferritin distribution and synthesis in sex-linked anemia.

The ferritin concentration of duodenum, liver, and spleen and the incorporation of L-leucine-3H into immunoprecipitated duodenal and liver ferritin was measured in genotypically normal (+/Y) mice and mice with sex-linked anemia (sla/Y), an X-linked recessive trait determined by a defect in intestinal iron absorption. Liver and splenic ferritin concentration was lower in sla/Y animals than in +/Y animals. Parenteral iron administration produced an increase in the duodenal, liver, and splenic ferritin concentration in both sla/Y and +/Y animals that was most striking in the case of the liver.

Quantitation of precipitating antibodies to certain soluble nuclear antigens in SLE.

Electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulfate was used to separate and quantitate the components of a washed immune precipitate. Serum was from patients with systemic lupus erythematosus known to have antibodies to soluble nuclear ribonucleoprotein (RNP) or to a soluble nuclear non-nucleic acid protein (Sm). Amounts of antibody that was predominantly IgG ranged from 0.

Nuclear deposits of immunoglobulins in skin of patients with systemic lupus erythematosus.

Skin nuclear speckled IgG deposition was noted in seven patients. The patients' clinical courses satisfied at least four of the preliminary criteria of the American Rheumatism Association for the diagnosis of systemic lupus erthematosus (SLE). Examination of approximately 700 additional biopsies from patiets with SLE, connective tissue and various skin diseases as well as normal individuals, failed to demonstrate similar nuclear immunoglobulin deposition.

The virtual absence of antigenic cross-reactivity between functionally distinct trout hemoglobins.

The antigenic properties of the hemoglobins of two species of rainbow trout, Salmo irideus and Salmo gairdneri, have been compared. Each of these species possess two different classes of hemoglobins, the members of the first of which have no Bohr effect while those of the second exhibit a pronounced pH dependence of their ligand affinities. The hemoglobins from these two species are antigenically indistinguishable.

Detection of immune complexes in mice infected with Mycobacterium lepraemurium.

A specific binding test was used to detect immune complexes containing antigens of Mycobacterium lepraemurium in the serum and tissues of infected mice. Complexes were precipitated by antiserum against immunoglogulin, free antigen removed by washing and the presence of bound antigen demonstrated by measurement of uptake of radioactively labelled specific antibody by the precipitate. Tests were done both with 125I-labelled FAB prepared from an immune from rabbit antiserum against M.

Radioimmunoassay of bovine heart protein kinase.

Immunization of guinea pigs with bovine cardiac cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.

Restriction of heterogeneity of goat antibodies specific for human hemoglobin SS.

Previously, we had reported the isolation of an antibody population (termed antiVal antibody) specific for the site of difference between human hemoglobin S(HbS)(beta-6Val) and hemoglobin A-1(BbA)(beta-6 glu). This population has a stoichiometry of reaction of unity in combining with HbS (alpha-beta-dimer) and shows no reaction with HbA. The combination of antiVal Fab fragments with HbS was found to be kinetically homogeneous and had a second order rate constant of 0.