Characterization of stress and protein turnover from protein overexpression in fed-batch E. coli cultures.

A structured kinetic model that accounts for proteolytic degradation due to recombinant protein overexpression is introduced and its performance evaluated by comparison with previously reported fed-batch experimental data. This mathematical model contains an additional pool for a generic key precursor (in our case phenylalanine), an improved IPTG transport term, a phenylalanine transport term, and a variable protein turnover expression that accounts for proteolytic activity. The model predictions concerning proteolytic activity, glucose level, and cell growth are in very good agreement with an amino acid depletion hypothesis.

Giardia lamblia: incorporation of free and conjugated fatty acids into glycerol-based phospholipids.

Giardia lamblia trophozoites are flagellated protozoa that inhabit the human small intestine, where they are exposed to various dietary lipids and fatty acids. It is believed that G. lamblia, which colonizes a lipid-rich environment of the human small intestine, is unable to synthesize phospholipids, long-chain fatty acids, and sterols de novo.

CD86 (B7-2), but not CD80 (B7-1), expression in the epidermis of transgenic mice enhances the immunogenicity of primary cutaneous Candida albicans infections.

Transgenic (Tg) mice whose epidermal keratinocytes constitutively overexpress either B7-1 (CD80) or B7-2 (CD86) exhibited exaggerated cutaneous delayed type hypersensitivity (DTH) to haptens compared to non-Tg mice. To determine whether enhanced DTH in these Tg mice is seen in response to cutaneous fungal infections, a primary infection with Candida albicans was established by inoculating this organism on the occluded skin of Tg and non-Tg mice. These infections resolved 7 days after removal of occlusive dressing in all three groups of mice, without evidence of exaggerated inflammation in either the Tg or non-Tg mice.

Epidermal growth factor protects against carbon tetrachloride-induced hepatic injury.

1. Epidermal growth factor (EGF) is known to protect the gastrointestinal tract against various noxious agents. Its potential value in preventing/ treating hepatic injury is, however, largely unexplored.

Longitudinal study of plasma HIV-1 RNA concentrations during the asymptomatic stage of HIV infection measured using AMPLICOR HIV monitor and NASBA HIV-1 RNA QT tests. ACCTES Association.

The predictive value of two methods for measuring HIV RNA concentration in plasma was assessed in relation to CD4 lymphocyte counts during the asymptomatic period of infection. The design was a retrospective longitudinal case-control study for a mean period of 60 months involving 20 asymptomatic patients included in the French National prospective survey. The CD4 counts in these patients during the last 36 months of the study were stable (non-progressors) or declined (progressors).

Pharmaceutical services in the Department of Veterans Affairs.

The status of pharmaceutical services in the Department of Veterans Affairs (VA) is described. The VA health care system is transforming itself from a hospital-based organization into 22 health care networks that emphasize primary and ambulatory care. The impact on VA pharmacy has been substantial.

Synaptophysin immunoreactivity in the aging rat pituitary gland.

The density of synaptophysin (SN)-immunoreactivity (IR) was examined in pituitary glands of aging male Sprague-Dawley rats. SN-IR was observed as dense dots among endocrine cells of the intermediate lobe, while the neural lobe contained numerous, highly dense immunopositive regions. Some anterior lobe secretory cells contained SN-IR within the cytoplasm, suggestive of the presence of the protein in secretory granules, but no dot-like staining was observed between endocrine cells of that region.

Laparoscopic resection of a mesenteric cyst: presentation of a new case.

Mesenteric cysts are rare abdominal tumors which develop as a result of embryonic defects of the lymphatics. Symptoms derived from this pathology are vague and nonspecific, being frequently abdominal pain, heartburn, and nausea. The treatment of these tumors is complete surgical excision.

Alterations in GAP-43-immunoreactive innervation in the aging rat pituitary.

The levels and distribution of the growth-associated protein, GAP-43, were examined in the pituitary glands of young and aging Sprague-Dawley rats, using immunohistochemical techniques on tissue sections and Western blot analyses. GAP-43-immunoreactive innervation was observed in sections in the intermediate and neural lobes of animals aged 8-15 months, while in the oldest rats studied (17 months), stained fibers were observed mainly in the neural, but not the intermediate lobe. Western blots revealed reduced levels of GAP-43 in samples from 15 month old animals, as compared to 12 month old rats, in the neurointermediate lobes.

Over-expression of Bcl-2 protects against apoptosis induced by the bioreductive cytotoxic drug SR4233 (Tirapazamine).

The human B-cell lymphoma cell line PW undergoes radiation-induced programmed cell death (PCD). Bcl-2 transfected PW cells, that overexpressed Bcl-2, were significantly more radioresistant than parental or neomycin control transfected PW cells. The viability of Bcl-2 transfected cells was significantly greater than that of parental PW cells treated with the bioreductive cytotoxin SR4233 under aerobic conditions.

HEMOCO: a French prospective study of hemophiliacs infected by human immunodeficiency virus type 1 (HIV-1).

HEMOCO is a multicenter prospective cohort set up in 1989 to monitor 407 French hemophiliacs infected by HIV-1 and recruited in 4 hemophilia treatment centers in the Paris region. As of 15 July 1995, 42% of the patients in the cohort had developed stage B HIV disease and 29% stage C disease (AIDS); 23.1% of the patients had died.

Fed-batch feeding and induction policies that improve foreign protein synthesis and stability by avoiding stress responses.

In this work, feeding policies aimed to avoid cellular stress responses as indicated by an increase in ATP-dependent proteolysis are tested. A set of experiments was carried out where glucose, IPTG (inducer), and phenylalanine (rate-limiting precursor) were added gradually in a fed-batch fashion. A significant increase in CAT activity was found compared with pulse-induction.

Regulation of pituitary beta-endorphin secretion in aging rats: in vitro responsiveness to dopamine.

The intermediate lobe of the mammalian pituitary is highly responsive to dopamine inhibition of beta-endorphin secretion. In this study, the ability of aged (12 months) intermediate lobes to respond to dopamine was compared to that of young (6 weeks) tissue, using a short-term in vitro incubation of isolated rat neurointermediate lobes, with measurement of peptide release by radioimmunoassay. Tissue from the aged rats released greater amounts of beta-endorphin peptide than amounts measured from young tissue at all time periods studied.

[Postsurgical gastric bezoar: presentation of a case of esophageal impaction].

A possible late sequel of gastric surgery is the formation of a phytobezoar in stomach. The phytobezoar can undergo fragmentation and the fragments can migrate causing obstructions. We present a case of proximal migration and esophageal obstruction.

Anaphylaxis to annatto dye: a case report.

Annatto dye is an orange-yellow food coloring extracted from the seeds of the tree Bixa orellana. It is commonly used in cheeses, snack foods, beverages, and cereals. Previously reported adverse reactions associated with annatto dye have included urticaria and angioedema.

Effect of circulating factors on vascular smooth muscle contraction and its calcium uptake in uremia.

Uremia is often associated with alterations in calcium metabolism and vascular smooth muscle function in hypertension and atherosclerosis. The ways in which these conditions inter-relate are not clearly understood. In order to study the possibility that circulating factors might influence smooth muscle function, experiments were performed on rat aortic strips.

[Prevalence of alcoholic diseases. At a teaching hospital of the Paris suburbs].

During the last 15 days of January 1989, the 757 patients admitted for more than 48 hours in any of the departments of Hôpital Beaujon (Clichy) were investigated for diseases that were certainly or probably related to alcoholism, and in order to detect drinkers at high risk, the amount of alcohol consumed in a week was recorded. The prevalence of alcohol-related diseases was 14.7 per cent (range: 12.

[Mucinous cystadenoma of the pancreas].

Pancreatic cystadenomas are rare neoplasms that represent 10% of all benign cystic lesions of the gland. Their biological features are not clearly delimited from cystadenocarcinoma, which has a similar aspect, making exhaustive histological study necessary after exeresis. A case is presented of mucinous cystadenoma of the body-tail of the pancreas that debuted as a painful abdominal mass in a patient with a history of acute pancreatitis, simulating a pseudocyst.

[Neurological manifestations in relation to vitamin E deficiency, caused by a defect of biliary acid synthesis].

A progressive neurological syndrome with cerebellar signs, abnormal proprioception, areflexia and Babinski response was observed in a child with chronic intestinal malabsorption. There was no ophtalmoplegia or retinitis pigmentosa. Electromyography and biopsy showed no axonopathy or myopathy.