The histone H4K20 methyltransferase SUV4-20H1/KMT5B is required for multiciliated cell differentiation in Xenopus.

H4 lysine 20 dimethylation (H4K20me2) is the most abundant histone modification in vertebrate chromatin. It arises from sequential methylation of unmodified histone H4 proteins by the mono-methylating enzyme PR-SET7/KMT5A, followed by conversion to the dimethylated state by SUV4-20H (KMT5B/C) enzymes. We have blocked the deposition of this mark by depleting Xenopus embryos of SUV4-20H1/H2 methyltransferases.

Transfer matrix method for light propagation in variable complex chiral media.

By taking a cholesteric liquid crystal (CLC) as an example and treating it as a multilayer stack of birefringent plates, we use a transfer matrix method to analyze light propagation in a common chiral medium in consideration of interlayer reflection and transmission. Based on the transfer matrix, the electric field distribution can be expressed in the form of linearly as well as circularly polarized components, so as to discuss the change of the polarization state of light in the transmission process. The transfer matrix of the same medium with different chirality can be converted by only changing the rotation matrix in the calculation process.

A systemic cell cycle block impacts stage-specific histone modification profiles during Xenopus embryogenesis.

Forming an embryo from a zygote poses an apparent conflict for epigenetic regulation. On the one hand, the de novo induction of cell fate identities requires the establishment and subsequent maintenance of epigenetic information to harness developmental gene expression. On the other hand, the embryo depends on cell proliferation, and every round of DNA replication dilutes preexisting histone modifications by incorporation of new unmodified histones into chromatin.

H4K20 Methylation Is Differently Regulated by Dilution and Demethylation in Proliferating and Cell-Cycle-Arrested Xenopus Embryos.

DNA replication during cell division leads to dilution of histone modifications and can thus affect chromatin-mediated gene regulation, raising the question of how the cell-cycle shapes the histone modification landscape, particularly during embryogenesis. We tackled this problem by manipulating the cell cycle during early Xenopus laevis embryogenesis and analyzing in vivo histone H4K20 methylation kinetics. The global distribution of un-, mono-, di-, and tri-methylated histone H4K20 was measured by mass spectrometry in normal and cell-cycle-arrested embryos over time.

Multifunctional and tunable trigate graphene metamaterial with "Lakes of Wada" topology.

Many plasmon-induced transparency (PIT) metamaterials previously reported had limited functions. Their tunabilities were realized by complex discrete structures, which greatly increased the difficulty and cost of device fabrication and adversely affected their resonance characteristics. It is an open question to adjust the Fermi levels of many graphene patterns with only a few in-plane electrodes.

Quantification of Proteins and Histone Marks in Drosophila Embryos Reveals Stoichiometric Relationships Impacting Chromatin Regulation.

Gene transcription in eukaryotes is regulated through dynamic interactions of a variety of different proteins with DNA in the context of chromatin. Here, we used mass spectrometry for absolute quantification of the nuclear proteome and methyl marks on selected lysine residues in histone H3 during two stages of Drosophila embryogenesis. These analyses provide comprehensive information about the absolute copy number of several thousand proteins and reveal unexpected relationships between the abundance of histone-modifying and -binding proteins and the chromatin landscape that they generate and interact with.

Magnetically tunable optical diffraction gratings based on a ferromagnetic liquid crystal.

Transmission optical diffraction gratings composed of periodic slices of a ferromagnetic liquid crystal and a conventional photoresist polymer are demonstrated. Dependence of diffraction efficiencies of various diffraction orders on an in-plane external magnetic field is investigated. It is shown that diffraction properties can be effectively tuned by magnetic fields as low as a few mT.

Coupling of Defect Modes in Cholesteric Liquid Crystals Separated by Isotropic Polymeric Layers.

Cholesteric liquid crystal structures with multiple isotropic defect layers exhibit localized optical modes (defect modes). Coupling effects between these modes were simulated using the finite difference time domain method. Analogous to the well-known result of the tight-binding approximation in solid state physics, splitting of the defect modes takes place, as soon as the structure contains more than one defect layer.

DNp73-induced degradation of tyrosinase links depigmentation with EMT-driven melanoma progression.

Melanoma is an aggressive cancer with poor prognosis, requiring personalized management of advanced stages and establishment of molecular markers. Melanomas derive from melanocytes, which specifically express tyrosinase, the rate-limiting enzyme of melanin-synthesis. We demonstrate that melanomas with high levels of DNp73, a cancer-specific variant of the p53 family member p73 and driver of melanoma progression show, in contrast to their less-aggressive low-DNp73 counterparts, hypopigmentation in vivo.

The Xenopus animal cap transcriptome: building a mucociliary epithelium.

With the availability of deep RNA sequencing, model organisms such as Xenopus offer an outstanding opportunity to investigate the genetic basis of vertebrate organ formation from its embryonic beginnings. Here we investigate dynamics of the RNA landscape during formation of the Xenopus tropicalis larval epidermis. Differentiation of non-neural ectoderm starts at gastrulation and takes about one day to produce a functional mucociliary epithelium, highly related to the one in human airways.

Laser direct writing of graphene nanostructures beyond the diffraction limit by graphene oxidation.

The fabrication ability of graphene nanostructures is the cornerstone of graphene-based devices, which are of particular interest because of their broad optical response and gate-tunable properties. Here, via laser-induced redox reaction of graphene and silica, we fabricate nano-scale graphene structures by femtosecond laser direct writing. The resolution of destructed graphene lines is far beyond the diffraction limit up to 100 nm with a precision as small as ± 7 nm.

Protection of the biconcave profile of human erythrocytes against osmotic damage by ultraviolet-A irradiation through membrane-cytoskeleton enhancement.

To perform various physiological functions, erythrocytes possess a unique biconcave shape provided by a special architecture of the membrane-skeleton system. In the present work, we use a simple irradiation method to treat human erythrocytes with 365 nm ultraviolet-A (UVA) light at the single-cell level . Depending on the irradiation dose, UVA show protection of the biconcave profile against the detrimental action of distilled water.

Multivalent binding of PWWP2A to H2A.Z regulates mitosis and neural crest differentiation.

Replacement of canonical histones with specialized histone variants promotes altering of chromatin structure and function. The essential histone variant H2A.Z affects various DNA-based processes via poorly understood mechanisms.

Brg1 chromatin remodeling ATPase balances germ layer patterning by amplifying the transcriptional burst at midblastula transition.

Zygotic gene expression programs control cell differentiation in vertebrate development. In Xenopus, these programs are initiated by local induction of regulatory genes through maternal signaling activities in the wake of zygotic genome activation (ZGA) at the midblastula transition (MBT). These programs lay down the vertebrate body plan through gastrulation and neurulation, and are accompanied by massive changes in chromatin structure, which increasingly constrain cellular plasticity.

Time-resolved photoluminescence of silicon microstructures fabricated by femtosecond laser in air.

Green photoluminescence (PL) from silicon microstructures fabricated by femtosecond laser in air was studied at different temperature by time-resolved spectroscopy. The PL decay profiles are well fitted by a stretched exponential function: I(t)=I(0)*exp[-(t/τ)β]. The dependence of the decay time constant τ and of the stretching index β on PL photon energy and on the temperature is investigated.

Potential oversummering and overwintering regions for the wheat stripe rust pathogen in the contiguous United States.

Epidemics of wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), are more frequent in the regions where Pst can oversummer and overwinter.

Characterizing inland Pacific Northwest American Viticultural Areas with geospatial data.

American Viticultural Areas are officially recognized appellations for wine grapes (Vitis vinifera L.). They represent not only geographic identification for growers, but also economic significance through price premiums for grapes from desirable appellations and wines sourcing grapes from such appellations.

Suv4-20h histone methyltransferases promote neuroectodermal differentiation by silencing the pluripotency-associated Oct-25 gene.

Post-translational modifications (PTMs) of histones exert fundamental roles in regulating gene expression. During development, groups of PTMs are constrained by unknown mechanisms into combinatorial patterns, which facilitate transitions from uncommitted embryonic cells into differentiated somatic cell lineages. Repressive histone modifications such as H3K9me3 or H3K27me3 have been investigated in detail, but the role of H4K20me3 in development is currently unknown.

Cardiac differentiation in Xenopus is initiated by mespa.

Aims: Future cardiac repair strategies will require a profound understanding of the principles underlying cardiovascular differentiation. Owing to its extracorporal and rapid development, Xenopus laevis provides an ideal experimental system to address these issues in vivo. Whereas mammalian MesP1 is currently regarded as the earliest marker for the cardiovascular system, several MesP1-related factors from Xenopus-mespa, mespb, and mespo-have been assigned only to somitogenesis so far.

Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species.

Ultraviolet (UV) light has a potent effect on biological organisms. Hemoglobin, an oxygen-transport protein, plays an irreplaceable role in sustaining life of all vertebrates. In this study we scrutinize the effects of ultraviolet irradiation (UVI) as well as visible irradiation on the fluorescence characteristics of bovine hemoglobin (BHb) in vitro.

Maternal Wnt/β-catenin signaling coactivates transcription through NF-κB binding sites during Xenopus axis formation.

Maternal Wnt/β-Catenin signaling establishes a program of dorsal-specific gene expression required for axial patterning in Xenopus. We previously reported that a subset of dorsally expressed genes depends not only on Wnt/β-Catenin stimulation, but also on a MyD88-dependent Toll-like receptor/IL1-receptor (TLR/IL1-R) signaling pathway. Here we show that these two signal transduction cascades converge in the nucleus to coactivate gene transcription in blastulae through a direct interaction between β-Catenin and NF-κB proteins.

Optical refocusing three-dimensional wide-field fluorescence lifetime imaging microscopy.

Three-dimensional fluorescence lifetime microscopy is achieved by combining wide-field fluorescence lifetime imaging with a remote optical refocusing method. As required for some applications in dynamic research for physics, chemistry, or biology, it is thereby not necessary to move the sample, i.e.

Stage-specific histone modification profiles reveal global transitions in the Xenopus embryonic epigenome.

Vertebrate embryos are derived from a transitory pool of pluripotent cells. By the process of embryonic induction, these precursor cells are assigned to specific fates and differentiation programs. Histone post-translational modifications are thought to play a key role in the establishment and maintenance of stable gene expression patterns underlying these processes.

Neutron optical beam splitter from holographically structured nanoparticle-polymer composites.

We report a breakthrough in the search for versatile diffractive elements for cold neutrons. Nanoparticles are spatially arranged by holographical means in a photopolymer. These grating structures show remarkably efficient diffraction of cold neutrons up to about 50% for effective thicknesses of only 200   μm.

Nonlinear spectrum broadening of femtosecond laser pulses in photorefractive waveguide arrays.

In photorefractive waveguide arrays, the process and extent of spectral broadening of femtosecond laser pulse caused by self-phase modulation are studied theoretically and experimentally. The threshold of self-phase modulation is more than two times larger than the common threshold in a bulk sample, which affects the extent of spectral broadening dramatically. The coupling length and the ratio between the common threshold and the input peak intensity of the femtosecond laser pulse are the two key parameters dominating these phenomena.