[Preparation of chiral monolithic column with covalently bonded cellulose and their application to rapid enantioseparation].

A chiral monolithic capillary column for rapid enantioseparation was prepared by covalently bonding of cellulose tris(4-methylbenzoate) (CTMB) on N-acryloxysuccinimide-based monolith. The preparation and derivatization conditions of the monolithic column were optimized. The successful grafting of CTMB was confirmed on the characterizations of the infrared spectrum and the cathodic electroosmotic flow (EOF).

[Evaluation of exposure and sensitization to house dust mites among rhinitis patients in the steppe environment of Inner Mongolia].

Objective: To evaluate the correlation of exposure and sensitization to house dust mites among rhinitis patients, and the consistency of skin prick test (SPT) and serum specific IgE (sIgE) in the detection of sensitization in a steppe environment of inner Mongolia.

Methods: Three hundreds and fourteen patients with rhinitis symptoms were recruited in this study in a hospital setting at Xilinhot City, inner Mongolia. All patients underwent medical history investigation, anterior rhinoscopy, and allergen tests in vivo (SPT) and in vitro (sIgE).

The increased expression of metabotropic glutamate receptor 5 in subventricular zone neural progenitor cells and enhanced neurogenesis in a rat model of intracerebral hemorrhage.

The metabotropic glutamate receptor 5 (mGluR5) is closely relative to the proliferation, survival, and differentiation of neural progenitor cells (NPCs). This study primarily examined the mGluR5 expression of NPCs in subventricular zone (SVZ) and the effects of mGluR5 on neurogenesis to intracerebral hemorrhage (ICH) rat. The experiment was designated as the following: (1) The ICH model was established by collagenase infusion into the right striatum of the rats, and the brain tissue was collected to assess the expression of mGluR5 in SVZ NPCs.

[Diagnostic use of D2-40 and annexin-1 in ependymal tumors].

Objective: To investigate the diagnostic significance of D2-40 and annexin-1 in the ependymal tumors.

Methods: To analyses the expression of D2-40, annexin-1, EMA and Ki-67 by immunohistochemistry in 52 cases of ependymal tumors (48 cases of ependymomas, 4 cases of choroid plexus papilloma) from Xuanwu Hospital from 2005 to 2009. Ten cases of corresponding normal brain tissue were also obtained as control.

Enantioselective toxic effects and biodegradation of benalaxyl in Scenedesmus obliquus.

Enantioselectivity in ecotoxicity and biodegradation of chiral pesticide benalaxyl to freshwater algae Scenedesmus obliquus was studied. The 96 h-EC(50) values of rac-, R-(-)-, S-(+)-benalaxyl were 2.893, 3.

(R,S)-2-chlorophenoxyl pyrazolides as novel substrates for improving lipase-catalyzed hydrolytic resolution.

The best reaction condition of Candida antartica lipase B as biocatalyst, 3-(2-pyridyl)pyrazole as leaving azole, and water-saturated methyl t-butyl ether as reaction medium at 45°C were first selected for performing the hydrolytic resolution of (R,S)-2-(4-chlorophenoxyl) azolides (1-4). In comparison with the kinetic resolution of (R,S)-2-phenylpropionyl 3-(2-pyridyl)pyrazolide or (R,S)-α-methoxyphenylacetyl 3-(2-pyridyl)pyrazolide at the same reaction condition, excellent enantioselectivity with more than two order-of-magnitudes higher activity for each enantiomer was obtained. The resolution was then extended to other (R,S)-3-(2-pyridyl)pyrazolides (5-7) containing 2-chloro, 3-chloro, or 2,4-dichloro substituent, giving good (E > 48) to excellent (E > 100) enantioselectivity.

Enantiomers of naringenin as pleiotropic, stereoselective inhibitors of cytochrome P450 isoforms.

Interactions between naringenin and the cytochrome P450 (CYP) system have been of interest since the first demonstration that grapefruit juice reduced CYP3A activity. The effects of naringenin on other CYP isoforms have been less investigated. In addition, it is well known that interactions with enzymes are often stereospecific, but due to the lack of readily available pure naringenin enantiomers, the enantioselectivity of its effects has not been characterized.

Quantitation of quinapril in human plasma by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with quinolone matrix additives.

The renin-angiotensin-aldosterone system (RAAS) is an essential body fluid maintenance system that controls pressure in the human body. The conversion of angiotensin I to angiotensin II by angiotensin-converting enzyme (ACE) is a key process in the RAAS because angiotensin II causes the vasoconstriction association with hypertension. Because of its effectiveness as an ACE blocker, quinipril is widely used for clinical treatment of hypertension and chronic congestive heart failure(.

[Development of Chinese version of children's fear survey schedule-dental subscale].

Objective: To establish the Chinese version of the modified children's fear survey schedule-dental subscale (CFSS-DS).

Methods: The original English version of CFSS-DS with facial image scale (FIS) was translated into Chinese, pre-tested and cross-culturally adapted. Subsequently the Chinese version schedule was randomly investigated among 367 children aged 5 - 12 years and their parents.

Rapid and selective determination of urinary lysozyme based on magnetic molecularly imprinted polymers extraction followed by chemiluminescence detection.

A rapid, low cost and selective chemiluminescence method coupled with magnetic molecularly imprinted polymers extraction was developed to detect lysozyme in human urine samples. Compared with traditional solid-phase extraction, this method could achieve selective extraction for the lysozyme, avoid the time consuming elution from a column or centrifugation steps, and then showed great potential in the high-throughput screening of clinical samples. The parameters affecting the performance of extraction and chemiluminescence were investigated.

Quantitation of the glutathione in human peripheral blood by matrix-assisted laser desorption ionization time-of-flight mass spectrometry coupled with micro-scale derivatization.

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been broadly applied to analyze high-molecular-weight compound (such as polymer or proteomic research) but seldom used for low-molecular-weight compound analysis. The objective of this study is the development of a simple analytical method for the determination of the concentration of tripeptide glutathione (GSH) by MALDI-TOF MS. Unfortunately, GSH could not be detected directly by MALDI-TOF MS.

Optically active mixed (phthalocyaninato)(porphyrinato) rare earth triple-decker complexes. Synthesis, spectroscopy, and effective chiral information transfer.

With the view to creating novel sandwich-type tetrapyrrole rare earth complexes toward potential applications in material science and chiral catalysis, two new optically active mixed (phthalocyaninato)(porphyrinato) rare earth triple-decker complexes with both (R)- and (S)-enantiomers [M(2)(Pc)(2)(TCBP)] {TCBP = Meso-tetrakis [3,4-(11,12:13,14-di(1',2'-naphtho)-1,4,7,10,15,18-hexaoxacycloeicosa-2,11,13-triene)-phenyl] porphyrinate; M = Eu (1), Y (2)} have been designed and prepared by treating optically active metal free porphyrin (R)-/(S)-H(2)TCBP with M(Pc)(2) in the presence of corresponding M(acac)(3)·nH(2)O (acac = acetylacetonate) in refluxing 1,2,4-trichlorobenzene (TCB). These novel mixed ring rare earth triple-decker compounds were characterized by a wide range of spectroscopic methods including MS, (1)H NMR, IR, electronic absorption, and magnetic circular-dichroism (MCD) spectroscopic measurements in addition to elemental analysis. Perfect mirror image relationship was observed in the Soret and Q absorption regions in the circular-dichroism (CD) spectra of the (R)- and (S)-enantiomers, indicating the optically active nature of these two mixed (phthalocyaninato)(porphyrinato) rare earth triple-decker complexes.

Human gingiva is another site of C-reactive protein formation.

Objectives: C-reactive protein (CRP) is primarily synthesized in the liver. It is hypothesized that human gingiva per se may produce CRP and its expression could be associated with IL-6. This study elucidated the CRP expression profile in human gingiva and its possible association with IL-6.

[Analysis of directional preponderance of caloric test in evaluation of vestibular dynamic compensation].

Objective: To analyze the value of directional preponderance (DP) in vestibular dynamic compensation evaluation.

Methods: One hundred fifty-five patients with sudden deafness accompanied vertigo were asked to accept routine vestibular tests including the spontaneous nystagmus (SN) and caloric test with video-nystagmography. DP, SN and unilateral weakness (UW) were selected as the observation parameters.

Enantioselective determination of trantinterol in rat plasma by ultra performance liquid chromatography-electrospray ionization mass spectrometry after derivatization.

An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the determination of trantinterol enantiomers in rat plasma. Diphenhydramine was employed as the internal standard. The plasma samples were prepared using liquid-liquid extraction with n-hexane-dichloromethane-isopropanol (20:10:1, v/v/v) as the extractant.

High-performance liquid chromatography-tandem mass spectrometry for the determination of pidotimod in human plasma and its application to a pharmacokinetic study.

A selective, rapid and sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the first time to determine pidotimod in human plasma and applied to a pharmacokinetic study. Diphenhydramine was used as the internal standard (I.S.

Synthesis and preclinical evaluation of [(11)C]PAQ as a PET imaging tracer for VEGFR-2.

Purpose: (R,S)-N-(4-Bromo-2-fluorophenyl)-6-methoxy-7-((1-methyl-3-piperidinyl)methoxy)-4-quinazolinamine (PAQ) is a tyrosine kinase inhibitor with high affinity for the vascular endothelial growth factor receptor 2 (VEGFR-2), which plays an important role in tumour angiogenesis. The aim of this work was to develop and evaluate in mice the (11)C-labelled analogue as an in vivo tracer for VEGFR-2 expression in solid tumours.

Methods: [(11)C]PAQ was synthesized by an N-methylation of desmethyl-PAQ using [(11)C]methyl iodide.

Development of an ICP-MS immunoassay for the detection of anti-erythropoietin antibodies.

A sandwich-type immunoassay linked with inductively coupled plasma mass spectrometry (ICP-MS) has been developed for the detection of anti-erythropoietin antibodies (anti-EPO Abs). Recombinant human erythropoietin (rhEPO) was immobilized on the solid phase to capture anti-rhEPO Abs specifically. After the immunoreactions with Au-labeled goat-anti-rabbit IgG, a diluted HNO(3) (2%) was used to dissociate Au nanoparticles which was then introduced to the ICP-MS for measurements.

Simultaneous determination of 11 active components in two well-known traditional Chinese medicines by HPLC coupled with diode array detection for quality control.

A simple and sensitive high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method was investigated for simultaneous determination of 11 components (chlorogenic acid, coptisine, epiberberine, jatrorrhizine, berberine, palmatine, baicalin, wogonoside, baicalein, wogonin and chrysin) in Qinhuanghouzheng (QHHZ) capsule and Xiaoerqingre (XEQR) tablet, for quality control of these two well-known traditional Chinese medicines (TCMs). The method was established using an Eclipse Plus C(18) (150 mm x 4.6 mm i.

Simultaneous quantification of enalapril and enalaprilat in human plasma by high-performance liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study.

A rapid, selective and sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed to simultaneously determine enalapril and enalaprilat in human plasma. With benazepril as internal standard, sample pretreatment involved in a one-step protein precipitation (PPT) with methanol of 0.2 ml plasma.

[Expression of nuclear export factor CRM1 and p27 in glioma].

Objective: To investigate the expression of nuclear export factor CRM1, Ser10-phosphorylated p27 and p27 in human gliomas.

Methods: The expression of CRM1, Ser10-phosphorylated p27 and p27 were investigated in 70 cases of human gliomas and 10 specimens of the normal brain tissue by immunohistochemical technique and Western blot.

Results: There were significant differences on the expression levels of CRM1, Ser10-phosphorylated p27 and p27 among normal brain tissue, gliomas of grades II and gliomas of grades III plus IV (P < 0.

Catalytic adsorptive stripping voltammetric measurements of trace vanadium at bismuth film electrodes.

Bismuth-coated glassy carbon electrodes have been successfully applied for catalytic adsorptive stripping voltammetric measurements of low levels of vanadium(V) in the presence of chloranilic acid (CAA) and bromate ion. The new protocol is based on the accumulation of the vanadium-chloranilic acid complex from an acetate buffer (pH 5.5) solution at a preplated bismuth film electrode held at -0.

Application of low temperature electrothermal vaporization ICP-AES for determination of refractory yttrium with 1-(2-pyridylazo)-2-naphthol as chemical modifier.

A low temperature electrothermal vaporization inductively coupled plasma atomic emission spectrometry (ETV-ICP-AES) method was developed for the determination of the refractory yttrium, using 1-(2-pyridylazo)-2-naphthol (PAN) as chemical modifier. The trace yttrium was vaporized as PAN complex into plasma from a graphite furnace at a comparatively low temperature of 1200 degrees C. The operation conditions were optimized, and the vaporization behavior of Y-PAN chelate and the main factors affecting the determination were investigated in detail.

Determination of total bilirubin in human serum by chemiluminescence from the reaction of bilirubin and peroxynitrite.

Peroxynitrous acid (ONOOH) was produced by the on-line reaction of acidified hydrogen peroxide with nitrite in a flow system, and peroxynitrite (ONOO(-)) was generated from ONOOH in NaOH solution. A weak chemiluminescent (CL) emission was observed due to the production of singlet oxygen ( O(2)1) during the decomposition of ONOO(-). Bilirubin and its conjugate were found to enhance the CL emission of O(2)1 in a suitable micellar medium.

Separation of polycyclic aromatic hydrocarbons by micellar electrokinetic chromatography with aqueous short-chain alcohol solvent.

Micellar electrokinetic capillary chromatography (MEKC) with aqueous organic solvent has been developed to separate polycyclic aromatic hydrocarbons (PAHs). Methanol, ethanol or propanol as an organic modifier was added to sodium dodecyl sulfate (SDS) micellar solution in order to increase the solubility of very hydrophobic solutes in mobile phase. Both methanol and ethanol can be used as co-solvents for the separation of PAHs.