Publications by authors named "R van Schendel"
Feruloylated side-chain oligosaccharide substituents are a distinctive feature of cereal grains' arabinoxylans (AX), but less is known about non-feruloylated oligosaccharide side-chain substituents. In this study we explored non-feruloylated disaccharide side-chains from corn (Zea mays L.) AX that had not been exposed to alkaline conditions and successfully isolated and unequivocally characterized the structure, α-d-xylopyranosyl-(1 → 3)-l-arabinose.
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- DNA double-strand breaks (DSBs) are critical to repair for maintaining genome stability, with different chromatin types potentially requiring distinct repair mechanisms.
- In a study involving Drosophila melanogaster, it was found that DSBs in facultative heterochromatin quickly move outside of specialized structures called polycomb bodies and this movement corresponds with a decrease in a specific histone mark, H3K27me3.
- The research indicates that the histone demethylase dUtx is essential for this process, as its absence disrupts both the movement of DSBs and the completion of repair via homologous recombination in heterochromatic regions.
Article Synopsis
- CRISPR technology helps scientists make precise changes in DNA, and understanding how cells repair broken DNA is important for this process.
- Two important tools, Cas9 and Cas12a, are used for editing genes in plants, and they work a little differently when they create DNA breaks.
- Both tools can cause mutations in similar ways, but they have different effects on how DNA is repaired, showing that either can be used effectively for engineering plants.
Background: Thinopyrum intermedium (Host) Barkworth & D.R. Dewey, or intermediate wheat grass (IWG), is being developed as the first widely-available perennial grain candidate.
View Article and Find Full Text PDFA practical and powerful approach for genome editing in plants is delivery of CRISPR reagents via transformation. The double-strand break (DSB)-inducing enzyme is expressed from a transferred segment of bacterial DNA, the T-DNA, which upon transformation integrates at random locations into the host genome or is captured at the self-inflicted DSB site. To develop efficient strategies for precise genome editing, it is thus important to define the mechanisms that repair CRISPR-induced DSBs, as well as those that govern random and targeted integration of T-DNA.
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