Protocol for the purification of the plastid-encoded RNA polymerase from transplastomic tobacco plants.

The plastid-encoded RNA polymerase (PEP) plays an essential role in the transcription of the chloroplast genome. Here, we present a strategy to purify the transcriptionally active protein complex from transplastomic tobacco (Nicotiana tabacum) lines in which one of the PEP core subunits is fused to an epitope tag. We describe experimental procedures for designing transformation constructs for PEP purification, selection, and analysis of transplastomic tobacco plants.

Posttraumatic stress disorder in disaster-exposed youth: examining diagnostic concordance and model fit using ICD-11 and DSM-5 criteria.

Background: Competing definitions of posttraumatic stress disorder (PTSD) have been proposed by ICD-11 and DSM-5; it is unclear which diagnostic model works best for children and adolescents. Although other studies have predicted the impact of these models by approximating the criteria using older measures, this study advances the research by comparing measures designed to assess ICD-11 and DSM-5 criteria in hurricane-exposed youth. This study evaluates ICD-11 and DSM-5 (both the standard and preschool-age) diagnostic models by identifying diagnostic rates, evaluating diagnostic concordance, investigating the predictive value of constructs associated with PTSD (demographics, disaster threat and exposure, functional impairment), and examining model fit.

Oxford Nanopore Technology-Based Identification of an Endosymbiosis in Microbial Keratitis.

(1) Background: Microbial keratitis is a serious eye infection that carries a significant risk of vision loss. spp. are known to cause keratitis and their bacterial endosymbionts can increase virulence and/or treatment resistance and thus significantly worsen the course of the disease.

Free-space optical communication link using a single Laguerre-Gaussian beam with tunable radial and azimuthal spatial indices generated by an integrated concentric circular antenna array.

We experimentally demonstrate a 10-Gbit/s free-space communication link using a single Laguerre-Gaussian (LG) beam with tunable radial and azimuthal modal indices generated by a photonic integrated circuit comprising two concentric uniform circular antenna arrays (UCAs). To tune the azimuthal modal indices ℓ of the generated beam, the azimuthal phase gradient inside each UCA is tuned. To tune the radial mode p of the generated beam, the amplitude ratio and phase difference between the two concentric UCA are tuned.

Test-retest reliability of behavioral and computational measures of advice taking under volatility.

The development of computational models for studying mental disorders is on the rise. However, their psychometric properties remain understudied, posing a risk of undermining their use in empirical research and clinical translation. Here we investigated test-retest reliability (with a 2-week interval) of a computational assay probing advice-taking under volatility with a Hierarchical Gaussian Filter (HGF) model.