Cold atmospheric plasma potentiates ferroptosis via EGFR(Y1068)-mediated dual axes on GPX4 among triple negative breast cancer cells.

Cold atmospheric plasma (CAP) has been proposed as an emerging onco-therapeutics that can specifically kill cancer cells without harming healthy cells. Here we explore its potency in triggering ferroptosis in transformed cells using triple negative breast cancer as the disease model. Through the whole transcriptome sequencing, mass spectrometry analysis, point mutation, and a series of and molecular assays, we identified two signaling axes centered at EGFR(Y1068), i.

Eurotium-Cristatum fermented black tea alleviates ulcerative colitis through the PPARγ-NF-κB signaling axis.

Ulcerative colitis (UC) is a chronic inflammatory bowel condition that significantly impairs patient quality of life and remains incurable. Effective dietary management is crucial for both prevention and treatment. This study investigates the effects and mechanisms of Eurotium cristatum-fermented black tea (FBT) in a dextran sulfate sodium (DSS)-induced UC mouse model using transcriptome sequencing, immunofluorescence, and flow cytometry.

Prediction of mortality in intensive care unit with short-term heart rate variability: Machine learning-based analysis of the MIMIC-III database.

Background: Prognosis prediction in the intensive care unit (ICU) traditionally relied on physiological scoring systems based on clinical indicators at admission. Electrocardiogram (ECG) provides easily accessible information, with heart rate variability (HRV) derived from ECG showing prognostic value. However, few studies have conducted a comprehensive analysis of HRV-based prognostic model against established standards, which limits the application of HRV's prognostic value in clinical settings.

Surface-Enhanced Raman Spectroscopy for the Detection of Reactive Oxygen Species.

Reactive oxygen species (ROS) play fundamental roles in various biological and chemical processes in nature and industries, including cell signaling, disease development and aging, immune defenses, environmental remediation, pharmaceutical syntheses, metal corrosion, energy production, etc. As such, their detection is of paramount importance, but their accurate identification and quantification are technically challenging due to their transient nature with short lifetimes and low steady-state concentrations. As a highly sensitive and selective analytical technique, surface-enhanced Raman spectroscopy (SERS) is promising for detecting ROS in real-time, enabling in situ monitoring of ROS-involved electrochemical and biochemical events with exceptional resolution.

An Experimental Study of the Flexural Bearing Capacity of Reinforced Concrete Beams Damaged by Explosions Using Piezoelectric Smart Aggregates.

Two identically sized RC beams were fabricated to investigate the effects of explosive loads on the flexural behaviour of Reinforced Concrete (RC) beams. One of the beams was subjected to an explosive load to induce post-explosion damage, and subsequently, both beams underwent flexural capacity testing. Integrating piezoelectric smart aggregates (SAas) within the beams facilitated continuous observation of the damage conditions, allowing for the assessment of internal concrete deterioration from explosive impacts to bending failures.