RNA-seq data and surprisal analysis of mutant and control strain of the green microalga during day/night cycles.

The data presented in this article are associated to the research article "Surprisal analysis of the transcriptomic response of the green microalga to the addition of acetate during day/night cycles" (Willamme et al., 2018) [1]. Here the RNA-seq data of the mutant, a null mutant of the isocitrate lyase gene, and its control are summarized and the FPKM values are listed.

Metabolomic analysis of the green microalga Chlamydomonas reinhardtii cultivated under day/night conditions.

Biomass composition of Chlamydomonas reinhardtii was studied during two consecutive cycles of 12h light/12h dark. As in our experimental conditions the two synchronized divisions were separated by 20h, it was possible to show that accumulation of dry weight, proteins, chlorophyll and fatty acids mainly depends on cell division, whereas starch accumulation depends on a circadian rhythm as reported previously. Our metabolomics analyses also revealed that accumulation of five (Ser, Val, Leu, Ile and Thr) of the nine free amino acids detected displayed rhythmicity, depending on cell division while Glu was 20-50 times more abundant than the other ones probably because this free amino acid serves not only for protein synthesis but also for biosynthesis of nitrogen compounds.

Lack of isocitrate lyase in Chlamydomonas leads to changes in carbon metabolism and in the response to oxidative stress under mixotrophic growth.

Isocitrate lyase is a key enzyme of the glyoxylate cycle. This cycle plays an essential role in cell growth on acetate, and is important for gluconeogenesis as it bypasses the two oxidative steps of the tricarboxylic acid (TCA) cycle in which CO₂ is evolved. In this paper, a null icl mutant of the green microalga Chlamydomonas reinhardtii is described.