Acetylation and silylation of piperidine solubilized sporopollenin from pollen of Typha angustifolia L.

Silyl and acetyl derivatives of sporopollenin from the pollen of Typha angustifolia L. were prepared. The derivatized products were readily soluble in piperidine-d11 and could be investigated employing one- and two-dimensional proton and carbon NMR (nuclear magnetic resonance) spectroscopy (1H,1H-COSY and 13C,1H-HETCOR techniques).

Continuous decomposition of sporopollenin from pollen of Typha angustifolia L. by acidic methanolysis.

Sporopollenin from the pollen of Typha angustifolia L. was exposed to a series of 36 subsequent acidic methanolysis procedures. The remaining decomposition products were investigated using several spectroscopic methods including Fourier transform infrared spectroscopy (FT-IR), solid state 13C nuclear magnetic resonance spectroscopy (13C-CPMAS-NMR) and X-ray photoelectron spectrometry (XPS).

Release of allergen-bearing cytoplasm from hydrated pollen: a mechanism common to a variety of grass (Poaceae) species revealed by electron microscopy.

Background: The release of submicronic particles from grass pollen after rainfall was suggested to be responsible for outbreaks of grass pollen asthma. Recently, we provided evidence for the release of respirable allergen-bearing particles from hydrated ryegrass (Lolium perenne ) pollen as a possible explanation for this phenomenon.

Objective: We investigated whether water-induced release of respirable allergen-bearing particles could be a mechanism common to several members of the sweet grass family Poaceae (Gramineae).

The nature of oxygen in sporopollenin from the pollen of Typha angustifolia L.

Native and peracetylated sporopollenin from the pollen of Typha angustifolia L. was investigated using several spectroscopic methods, inducing Fourier transform infrared spectroscopy (FTIR), solid-state 13C-nuclear magnetic resonance spectroscopy (13C-NMR) and X-ray photoelectron spectrometry (XPS). Interpretation of the experimental data shows that the greater part of oxygen found in sporopollenin originates from hydroxyl groups and must be derived from aliphatics and not from aromatics.

A new protocol to prepare dry plant specimens for electron microscopy and immunocytochemistry.

Samples from dry kidney bean cotyledons were fixed in acrolein vapor and embedded in Lowicryl K4M using a modified dehydration and embedding technique. The preservation of morphology as investigated by transmission electron microscopy was excellent showing desiccated cells and organelles in life-like preservation. Postembedding immunogold labeling for kidney bean purple acid phosphatase (KbPAP) showed association of kbPAP with ribosome-rich areas of the cytoplasm.