Understanding anxiety experienced by young males with Duchenne muscular dystrophy: a qualitative focus group study.

In this multi-methods study we explored the characteristics, causes and impact of anxiety in Duchenne muscular dystrophy (DMD) from the perspective of young males with DMD and their parents. Eight young males with DMD (7-18 years) and 14 parents participated in separate focus groups. Perspectives on anxiety were explored using semi-structured interview schedules.

Acridine orange detection of Plasmodium falciparum malaria: relationship between sensitivity and optical configuration.

Blood samples collected from five volunteers participating in a P. falciparum infectivity trial were examined to determine the efficacy of the acridine orange technique. Several lens configurations were tested for efficiency in the diagnosis of malaria using this system.

New dip-and-read test for determining leukocytes in urine.

A new reagent strip for the determination of leukocytes in urine (LEUKOSTIX; Ames) is described. The test is based on the esterase activity in leukocytes as a marker. Upon contact between the reagent matrix and a urine containing leukocytes, an amino acid ester is hydrolyzed by the esterase to its corresponding alcohol.

High frequency of c-K-ras activation in 3-methylcholanthrene-induced mouse thymomas.

Thymic lymphomas were induced in RJF mice by percutaneous application of 3-methylcholanthrene (MCA). DNAs from 83% of the tumors analyzed contained a transforming c-K-ras gene. The high frequency of c-K-ras oncogene activation in response to MCA seems to favor the concept that the activation of c-K-ras is related to the specificity of the mutagenic effect of MCA.