Conversion of mannose to fructose by immobilized mannose isomerase from Pseudomonas cepacia.

The enzyme mannose isomerase (EC 5.3.1.

Regulation of beta-1, 4-Glucosidase Expression by Candida wickerhamii.

Candida wickerhamii NRRL Y-2563 expressed beta-glucosidase activity (3 to 8 U/ml) constitutively when grown aerobically in complex medium containing either glycerol, succinate, xylose, galactose, or cellobiose as the carbon source. The addition of a high concentration of glucose (>75 g/liter) repressed beta-glucosidase expression (<0.3 U/ml); however, this yeast did produce beta-glucosidase when the initial glucose concentration was View Article and Find Full Text PDF

Induction of NADPH-linked D-xylose reductase and NAD-linked xylitol dehydrogenase activities in Pachysolen tannophilus by D-xylose, L-arabinose, or D-galactose.

Considerable interest in the D-xylose catabolic pathway of Pachysolen tannophilus has arisen from the discovery that this yeast is capable of fermenting D-xylose to ethanol. In this organism D-xylose appears to be catabolized through xylitol to D-xylulose. NADPH-linked D-xylose reductase is primarily responsible for the conversion of D-xylose to xylitol, while NAD-linked xylitol dehydrogenase is primarily responsible for the subsequent conversion of xylitol to D-xylulose.

Characterization of cellobiose fermentations to ethanol by yeasts.

Twenty-two different yeasts were screened for their ability to ferment both glucose and cellobiose. The fermentation characteristics of Candida lusitaniae (NRRL Y-5394) and C. wickerhamii (NRRL Y-2563) were selected for further study because their initial rate of ethanol production from cellobiose was faster than the other test cultures.

Biomass conversion: fermentation chemicals and fuels.

Recent events clearly establish that petroleum can no longer be relied upon as a stable, economical raw material for energy and industrial chemicals. Plant biomass is currently being evaluated as a desirable alternative raw material to petroleum because of renewability and abundance. The most abundant form of biomass on the planet earth is lignocellulose which is composed of cellulose, hemicellulose, and lignin.