Publications by authors named "R Villanueva-Romero"
Several neuropeptides present in bone tissues, produced by nerve fibers and bone cells, have been reported to play a role in regulating the fine-tuning of osteoblast and osteoclast functions to maintain bone homeostasis. This study aims to characterize the influence of the neuropeptide vasoactive intestinal peptide (VIP) on the differentiation process of human mesenchymal stem cells (MSCs) into osteoblasts and on their anabolic function. We describe the mRNA and protein expression profile of VIP and its receptors in MSCs as they differentiate into osteoblasts, suggesting the presence of an autocrine signaling pathway in these cells.
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- The study investigates the expression of vasoactive intestinal peptide (VIP) and its receptors VPAC1 and VPAC2 in patients with immune-mediated inflammatory disorders (IMID) to understand their shared mechanisms.
- Results show that IMID patients had significantly higher levels of VIP and greater VPAC2 expression compared to healthy controls, making these markers potential indicators for diagnosing IMIDs.
- Treatment with biological therapies, like anti-TNFα and anti-IL12/23, influenced serum VIP levels, and specific miRNA signatures were linked to VIP and VPAC2 levels, suggesting a deeper connection between these markers and IMIDs.
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- Naïve CD4 T cells require different signals during activation to elicit a proper immune response, and this study focused on their behavior after stimulation for 14 days.
- The findings revealed that activated CD4 T cells showed increased glucose needs and developed a pathogenic Th17 profile, indicating a potential flexibility towards a Th17/1 phenotype, while regulatory T cells did not appear functional.
- The study also highlighted the role of the vasoactive intestinal peptide (VIP) system, which was found to modulate gene expressions related to Th17 stabilization and plasticity, underlining VIP's importance in T cell responses in various conditions.
We aimed to evaluate the direct action of VIP on crucial molecules involved in human osteoclast differentiation and function. We also investigated the relationship between VIP serum levels and bone remodeling mediators in early arthritis patients. The expression of VIP receptors and osteoclast gene markers in monocytes and in vitro differentiated osteoclasts was studied by real-time PCR.
View Article and Find Full Text PDFProteomic Analysis of Synovial Fibroblasts and Articular Chondrocytes Co-Cultures Reveals Valuable VIP-Modulated Inflammatory and Degradative Proteins in Osteoarthritis.
Int J Mol Sci
June 2021
Osteoarthritis (OA) is the most common musculoskeletal disorder causing a great disability and a reduction in the quality of life. In OA, articular chondrocytes (AC) and synovial fibroblasts (SF) release innate-derived immune mediators that initiate and perpetuate inflammation, inducing cartilage extracellular matrix (ECM) degradation. Given the lack of therapies for the treatment of OA, in this study, we explore biomarkers that enable the development of new therapeutical approaches.
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