Overall study of the in vitro plasma clotting system in an invertebrate, Liocarcinus puber (Crustacea Decapoda): considerations on the structure of the Crustacea plasma fibrinogen in relation to evolution.

An overall study of the in vitro plasma coagulation system in the crab Liocarcinus puber has been carried out using various analytical methods, namely thromboelastography, spectrophotometrical examination, and a new one based on changes of the mechanical impedance of the developing clot. From the results reported here the clotting pattern in this species appears surprisingly complex for an invertebrate and unexpectedly closer to that of the vertebrates. Indirect evidences suggest that the fibrinogen polypeptide chains in this species and very likely in the other crustacean, are very different from those of the vertebrates.

Cross induced coagulations between human and crustacean clotting factors. Considerations on the clotting processes.

1. Cross induced coagulations show that human factor XIII and crustacean coagulin are to some extent functionally equivalent and may be substituted for each other. 2.

[Coagulation of hemolymph in Crustacea. Electrophoretic demonstration of a plasmatic component in the course of hemostasis in a group A crustacean (Cancer pagurus)].

Coagulation assays in vitro of plasmatic extract from Cancer pagurus reveal the existence of a plasmatic coagulation in this species which was considered as yet as lacking a such process. Electrophoretical analysis of this extract before and after coagulation shows the presence of a plasmatic component probably involved in the formation of the clot.

[Coagulation of the hemolymph in Macropipus puber (L)., Crustacea, Decapoda. Electrophoretic demonstration of a plasma component intervening in hemostasis].

Electrophoretical and immuno-electrophoretical analysis of plasma, serum and some plasmatic extracts of Macropipus puber (L). have evidenced the presence in the plasma of a component absent in the serum. This component which disappears from the plasmatic extracts after their coagulation must therefore plays a role in the clotting processes.

[Hemolymph coagulation in Macropipus puber (L.) Crustacea Decapoda. Respective roles of hemolymph and plasma].

The process of coagulation of the hemolymph in Macropipus puber (L.) Crustacea Decapod depends on one or several plasmatic factors and on a thermo-labile cellular factor which does not seem to be specific. The existence of these factors has been demonstrated.