Publications by authors named "R Vazquez-Duhalt"
Article Synopsis
- Nanotechnology, particularly silver nanoparticles (AgNPs), shows promise in improving cancer treatments, especially in overcoming chemotherapy resistance and reducing side effects from toxic drugs.
- Research demonstrated that combining AgNPs with the drug tamoxifen leads to a synergistic effect that not only increases cell death in breast cancer cells but also prevents their migration and boosts reactive oxygen species production.
- Despite these effects, the combination of AgNPs and tamoxifen exhibited minimal genotoxicity, and both types of breast cancer cells showed heightened expression of genes linked to cell death and oxidative stress, indicating potential for further exploration in cancer therapy.
An accurate spectrophotometric method for chitosan quantification.
Biol Methods Protoc
November 2023
Article Synopsis
- Chitosan is a biopolymer derived from chitin, widely found in nature, with diverse applications across biomedical, environmental, and industrial sectors.
- A new spectrophotometric method has been developed to accurately measure chitosan concentration.
- This method involves deamination of chitosan using nitrite in acidic conditions and subsequently determining carbohydrates using the anthrone reagent.*
Article Synopsis
- Copper oxide nanoparticles (CuO NPs) are commonly used in many products but can pose risks, prompting a study on their cytotoxic effects on kidney MDCK and liver AML-12 cells.
- The study found that CuO NPs decreased cell viability in a concentration-dependent manner and triggered an increase in reactive oxygen species, leading to cell cycle arrest and distinctive forms of cell death in both cell lines.
- This research highlights the need for further investigation into the mechanisms of toxicity from CuO NPs and copper ions in excretory system cells, which could inform safer usage guidelines and future nanotoxicological studies.
Background: Breast cancer is the most common malignant tumor disease and the leading cause of female mortality. The evolution of nanomaterials science opens the opportunity to improve traditional cancer therapies, enhancing therapy efficiency and reducing side effects.
Methods And Major Results: Herein, protein cages conceived as enzymatic nanoreactors were designed and produced by using virus-like nanoparticles (VLPs) from Brome mosaic virus (BMV) and containing the catalytic activity of glucose oxidase (GOx) enzyme.
Copper oxide nanoparticles (CuO-NPs) were functionalized with specific antibodies to target their antibacterial activity against Gram-positive or Gram-negative bacteria. The CuO-NPs were covalently functionalized to cover their surface with specific antibodies. The differently prepared CuO-NPs were characterized by X-ray diffraction, transmission electron microscopy and dynamic light scattering.
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