Ice cream structure modification by ice-binding proteins.

Ice-binding proteins (IBPs), also known as antifreeze proteins, were added to ice cream to investigate their effect on structure and texture. Ice recrystallization inhibition was assessed in the ice cream mixes using a novel accelerated microscope assay and the ice cream microstructure was studied using an ice crystal dispersion method. It was found that adding recombinantly produced fish type III IBPs at a concentration 3 mg·L made ice cream hard and crystalline with improved shape preservation during melting.

The transcriptional repressor domain of Gli3 is intrinsically disordered.

The transcription factor Gli3 is acting mainly as a transcriptional repressor in the Sonic hedgehog signal transduction pathway. Gli3 contains a repressor domain in its N-terminus from residue G106 to E236. In this study we have characterized the intracellular structure of the Gli3 repressor domain using a combined bioinformatics and experimental approach.

From chemical embryology to nucleosome patterning - an interview with Roumen G. Tsanev.

Roumen Tsanev was a prominent Bulgarian scientist whose pioneering ideas about the role for chromatin in cell differentiation and development led him to propose the first hypothesis for epigenetic information based on a histone code. To test experimentally his ideas, Dr.Tsanev explored nucleosome structure and heterogeneity and generated seminal data on nucleosome segregation in replicating chromatin.

Identification of the gene transcription repressor domain of Gli3.

Gli transcription factors are downstream targets of the Hedgehog signaling pathway. Two of the three Gli proteins harbor gene transcription repressor function in the N-terminal half. We have analyzed the sequences and identified a potential repressor domain in Gli2 and Gli3 and have tested this experimentally.

Molecular mechanisms of cancer cells survival.

It is of great interest for cancer therapy to elucidate and overcome the mechanisms that cancer cells develop to fight back the defense systems of the organism and anticancer treatments. The anticancer defense of the organism consists of four processes: 1. Activity of the cellular immunity; 2.